An aptamer-based biosensor for detection of doxorubicin by electrochemical impedance spectroscopy
An aptamer-based biosensor for detection of doxorubicin by electrochemical impedance spectroscopy
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DOI：
10.1007/s00216-017-0786-8
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发表时间：
2018-02-01
影响因子：
4.3
通讯作者：
Beckmann, Dieter
中科院分区：
文献类型：
--
作者：
Bahner, Nicole;Reich, Peggy;Beckmann, Dieter
研制了一种基于适体的生物传感器，用于阿霉素的电化学阻抗谱检测。阿霉素及其14-脱羟基的柔红霉素是经常用于癌症治疗的蒽环类药物。由于它们的致突变和心脏毒性作用，在地下水中进行检测是可取的。我们研制了一种以柔红霉素结合适体为生物识别元件的生物传感器。将核酸适体与巯基己醇共固定在金表面，得到了密度为1.3×10(13)+/-2.4×10(12)个/cm(2)的核酸适体。通过电化学阻抗谱检测阿霉素与固定化适配子的结合。其原理是由于阿霉素与固定化适配子的结合而抑制了电极与溶液中铁/铁氰化铁之间的电子转移。在31~125nM范围内，电荷转移电阻(R(Ct))与阿霉素浓度呈线性关系，表观结合常数为nM，检出限为28 nM。该方法具有灵敏度高、选择性好、传感器结构简单等优点，在环境监测中显示出良好的应用潜力。
An aptamer-based biosensor was developed for the detection of doxorubicin using electrochemical impedance spectroscopy. Doxorubicin and its 14-dehydroxylated version daunorubicin are anthracyclines often used in cancer treatment. Due to their mutagenic and cardiotoxic effects, detection in groundwater is desirable. We developed a biosensor using the daunorubicin-binding aptamer as biological recognition element. The aptamer was successfully co-immobilized with mercaptohexanol on gold and a density of 1.3*10(13) +/- 2.4*10(12) aptamer molecules per cm(2) was achieved. The binding of doxorubicin to the immobilized aptamer was detected by electrochemical impedance spectroscopy. The principle is based on the inhibition of electron transfer between electrode and ferro-/ferricyanide in solution caused by the binding of doxorubicin to the immobilized aptamer. A linear relationship between the charge transfer resistance (R (ct) ) and the doxorubicin concentration was obtained over the range of 31 nM to 125 nM doxorubicin, with an apparent binding constant of 64 nM and a detection limit of 28 nM. With the advantages of high sensitivity, selectivity, and simple sensor construction, this method shows a high potential of impedimetric aptasensors in environmental monitoring.