Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis
Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis
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DOI：
10.1002/1873-3468.13375
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发表时间：
2019-05-01
期刊：
影响因子：
3.5
通讯作者：
Mande, Shekhar C.
中科院分区：
文献类型：
--
作者：
Kumar, Ashwani;Phulera, Swastik;Mande, Shekhar C.
结核分枝杆菌的转录因子Rv 0081控制低氧基因的表达，并在结核病（TB）感染的潜伏期作为一个调节中心。我们在这里报告的晶体结构Rv 0081在2.9埃分辨率揭示它属于著名的ArsR/SmtB家族蛋白。然而，与该家族中的其他成员不同，Rv 0081既没有金属结合结构域，也没有Cys残基，这表明了基因调控的替代机制。我们的结构和生化分析表明，自我调节的DNA序列的识别和合理的机制，在结核病感染的潜伏期的Rv 0081的调节的分子基础。数据库结构数据可在蛋白质数据库中以登录号-
The transcription factor Rv0081 of Mycobacterium tuberculosis controls hypoxic gene expression and acts as a regulatory hub in the latent phase of tuberculosis (TB) infection. We report here the crystal structure of Rv0081 at 2.9 angstrom resolution revealing that it belongs to the well-known ArsR/SmtB family proteins. However, unlike other members in this family, Rv0081 has neither a metal-binding domain nor does it possess Cys residues, suggesting an alternate mechanism of gene regulation. Our structural and biochemical analyses suggest the molecular basis for the recognition of self-regulatory DNA sequences and a plausible mechanism of regulation of Rv0081 in the latent phase of TB infection. Database Structural data are available in the Protein Data Bank under the accession number -