Synthesis of [35S]thiophosphoryl adenylic acid, utilizing a general procedure for [35S]thiophosphoryl chloride production.
Synthesis of [35S]thiophosphoryl adenylic acid, utilizing a general procedure for [35S]thiophosphoryl chloride production.
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[35S]硫代磷酰腺苷酸的合成，采用[35S]硫代磷酰氯生产的一般程序。
DOI：
10.1006/abio.1993.1094
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发表时间：
1993
影响因子：
2.9
通讯作者：
Keenan,RW
中科院分区：
文献类型：
--
作者：
Slama,JT;Simmons,AM;Hernandez,TM;Keenan,RW
当这些材料一起加热时，元素[35 S]硫显示出与硫代磷酰氯的硫平衡。这种同位素交换反应是高比活度[35 S] PSCl 3的方便、微量合成的基础。[35 S]硫代磷酰氯除通过定制合成外，不可商购。将标记的硫代磷酰氯用于制备[35 S]腺苷5′-硫代磷酸酯的新方法。这种同位素交换方法应找到广泛的应用在许多放射性标记的硫代磷酰酯的合成，利用PSCl 3作为源的硫代磷酰基团。
Elemental [35S]sulfur was shown to equilibrate with the sulfur of thiophosphoryl chloride when these materials are heated together. This isotopic exchange reaction is the basis of a convenient, microscale synthesis of high specific activity [35S]PSCl3. [35S]Thiophosphoryl chloride is otherwise not commercially available except through custom synthesis. The labeled thiophosphoryl chloride was used in a novel procedure for the preparation of [35S]adenosine 5′-phosphorothioate. This isotopic exchange method should find wide application in the synthesis of many radiolabeled thiophosphoryl esters which utilize PSCl3as the source of the thiophosphoryl group.