Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits
Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits
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DOI：
10.1111/j.1365-2672.2004.02435.x
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Sakthivel, N
中科院分区：
文献类型：
--
作者：
Kumar, RS;Ayyadurai, N;Sakthivel, N
目的：研究一株广谱拮抗荧光假单胞菌PUPa 3的抑菌活性和植物有益性状。方法与结果：从水稻根际土壤中分离得到一株PUPa 3，经生化试验和16 S rDNA序列比较，鉴定为铜绿假单胞菌（Pseudomonasaeruginosa）。该细菌对植物病原真菌具有广谱抗真菌活性。利用核磁共振（NMR）和质谱（MS）对PUPa 3的抗真菌代谢产物进行了提取、纯化和表征。测定了PUPa 3中吲哚-3-乙酸（IAA）、铁载体、磷酸酶和蛋白酶的产生。结论：利用NMR和MS技术鉴定了菌株PUPa 3产生的抗真菌代谢产物为吩嗪-1-甲酰胺（PCN）。菌株PUPa 3对多种植物病原真菌具有广谱抗真菌活性。这种细菌也表现出几种植物生长促进性状，但没有表现出的特性归因于有害的rhizobacteries.Significance和影响的研究：本研究报告的生产PCN以及IAA的第一次由一个sacetitic铜绿假单胞菌菌株PUPa 3。由于产生铁载体、生长激素、蛋白酶和磷酸酶及其固有的杀真菌潜力，该菌株可用作生物肥料和对抗感染水稻、花生、烟草、辣椒、芒果、甘蔗、茶、棉花和香蕉的一系列植物病原真菌的拮抗剂。
Aim: To study the antifungal activity and plant beneficial traits of a broad-spectrum antagonistic fluorescent pseudomonad strain, PUPa3.Methods and Results: Strain PUPa3 was isolated from the rhizosphere soil of rice and identified as Pseudomonas aeruginosa on the basis of biochemical tests and by comparison of 16S rDNA sequences. This bacterium exhibits a broad-spectrum antifungal activity towards phytopathogenic fungi. The antifungal metabolite by PUPa3 was extracted, purified and characterized using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). Production of indole-3-acetic acid (IAA), siderophores, phosphatase and protease in PUPa3 was determined. Strain PUPa3 did not produce hydrogen cyanide, cellulase and pectinase.Conclusion: The antifungal metabolite produced by PUPa3 has been identified as phenazine-1-carboxamide (PCN) on the basis of NMR and MS data. Strain PUPa3 showed a broad-spectrum antifungal activity towards a range of phytopathogenic fungi. This bacterium also showed several plant growth-promoting traits but did not show the traits attributed to deleterious rhizobacteria.Significance and Impact of the Study: Present study reports the production of PCN as well as IAA for the first time by a saprophytic P. aeruginosa strain PUPa3. Because of the production of siderophore, growth hormone, protease and phosphatase and its innate fungicidal potential, this strain can be used as biofertilizer and antagonist against a range of phytopathogenic fungi that infect rice, groundnut, tobacco, chili, mango, sugarcane, tea, cotton and banana.