PRODUCTION OF AN EPIDERMAL GROWTH-FACTOR RECEPTOR-RELATED PROTEIN
PRODUCTION OF AN EPIDERMAL GROWTH-FACTOR RECEPTOR-RELATED PROTEIN
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DOI：
10.1126/science.6324343
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
SPIESS, J
中科院分区：
文献类型：
--
作者：
WEBER, W;GILL, GN;SPIESS, J
培养的人表皮样癌A431细胞产生一种可溶性105千道尔顿的蛋白，根据表皮生长因子（EGF）结合、EGF受体单克隆和多克隆抗体识别、氨基末端序列分析和碳水化合物含量的标准，该蛋白与EGF受体的细胞表面结构域有关。高产量和生物合成标记的发现，这种105千道尔顿蛋白的特异性活性超过了完整的受体，这表明它不是来自膜结合的成熟受体，而是由细胞单独产生的。这些细胞分别合成插入细胞膜的EGF受体和分泌的EGF受体相关蛋白。
Human epidermoid carcinoma A431 cells in culture produce a soluble 105-kilodalton protein which, by the criteria of epidermal growth factor (EGF) binding, recognition by monoclonal and polyclonal antibodies to the EGF receptor, amino-terminal sequence analysis and carbohydrate content, is related to the cell surface domain of the EGF receptor. The high rate of production and the finding that with biosynthetic labeling the specific activity of this 105-kilodalton protein exceeds that of the intact receptor indicate that it is not derived from membrane-bound mature receptor but is separately produced by the cell. These cells separately synthesize an EGF receptor that is inserted into the membrane and an EGF receptor-related protein that is secreted.