Influenza virus.
Influenza virus.
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DOI：
10.1002/0471142735.im1911s42
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发表时间：
2001-05-01
影响因子：
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通讯作者：
Bender, B S
中科院分区：
文献类型：
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作者：
Cottey, R;Rowe, C A;Bender, B S
本单元包含几种用流感病毒感染小鼠的方法。它还包括在鸡蛋中繁殖流感病毒，定量病毒滴度（在组织培养基和流感感染的小鼠血清中）以及采用人类流感分离株在小鼠中生长所需的方案。还包括测量50%小鼠致死剂量的方法。最后，提供了用于从脾细胞前体产生抗流感细胞毒性T淋巴细胞（CTL）并在用流感病毒对小鼠进行呼吸道攻击后收获肺CTL的方案。
This unit contains several methods for infecting mice with influenza virus. It also includes protocols needed to propagate influenza virus in hen eggs, quantitate virus titers (in tissue culture medium and in influenza-infected mouse serum), and adopt human isolates of influenza for growth in mice. Methods for measuring the 50% mouse lethal dose are also included. Finally, protocols for generating anti-influenza cytotoxic T lymphocytes (CTL) from splenocyte precursors and harvesting pulmonary CTL following respiratory tract challenge of mice with influenza virus are provided.