Correlative BOLD MR imaging of stages of synovitis in a rabbit model of antigen-induced arthritis
Correlative BOLD MR imaging of stages of synovitis in a rabbit model of antigen-induced arthritis
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DOI：
10.1007/s00247-011-2194-0
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发表时间：
2012-01-01
影响因子：
2.3
通讯作者：
Salter, Robert B.
中科院分区：
文献类型：
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作者：
Doria, Andrea S.;Crawley, Adrian;Salter, Robert B.
为了评价血氧水平依赖性（BOLD）MRI检测炎症性关节炎兔膝关节滑膜周围间隙变化的可行性，将9只兔膝关节分别关节内注射白蛋白（n = 9）和生理盐水（n = 6），并将6只兔膝关节内注射白蛋白（n = 10）和生理盐水（n = 10），观察其关节周围间隙变化。或不注射（对照膝，n = 9）。除了两只分别在实验第7天和第21天意外死亡的兔子（白蛋白注射，n = 2个膝关节;盐水注射，n = 2个膝关节）外，所有其他动物在诱导关节炎后第0、1、7、14、21和28天用BOLD MRI扫描。T2* 加权梯度回波MRI进行了交替30 s的常氧/高氧。将BOLD MRI测量结果与临床、实验室和组织学标记物进行比较，白蛋白注射膝关节的激活体素百分比显著高于对侧盐水注射膝关节（P = 0.04）。对于注射白蛋白的膝关节（P < 0.05）和不同类别的膝关节（P = 0.009），激活的BOLD体素的百分比随着时间的推移而变化。描绘了白蛋白和生理盐水注射膝关节随时间推移的开启和关闭BOLD差异的二次曲线（白蛋白注射，P = 0.047;生理盐水注射，P = 0.009）。注射白蛋白和注射生理盐水的膝关节之间的滑膜组织学评分有显著差异的趋势，仅在急性评分中观察到（P = 0.07）。作为概念的证明，BOLD MRI可以描述实验性关节炎进展过程中的滑膜周围变化。
Because of the ability of blood-oxygen-level-dependent (BOLD) MRI to assess blood oxygenation changes within the microvasculature, this technique holds potential for evaluating early perisynovial changes in inflammatory arthritis.To evaluate the feasibility of BOLD MRI to detect interval perisynovial changes in knees of rabbits with inflammatory arthritis.Rabbit knees were injected with albumin (n = 9) or saline (n = 6) intra-articularly, or were not injected (control knees, n = 9). Except for two rabbits (albumin-injected, n = 2 knees; saline-injected, n = 2 knees) that unexpectedly died on days 7 and 21 of the experiment, respectively, all other animals were scanned with BOLD MRI on days 0, 1, 7, 14, 21 and 28 after induction of arthritis. T2*-weighted gradient-echo MRI was performed during alternate 30 s of normoxia/hyperoxia. BOLD MRI measurements were compared with clinical, laboratory and histological markers.Percentage of activated voxels was significantly greater in albumin-injected knees than in contralateral saline-injected knees (P = 0.04). For albumin-injected knees (P < 0.05) and among different categories of knees (P = 0.009), the percentage of activated BOLD voxels varied over time. A quadratic curve for on-and-off BOLD difference was delineated for albumin- and saline-injected knees over time (albumin-injected, P = 0.047; saline-injected, P = 0.009). A trend toward a significant difference in synovial histological scores between albumin-injected and saline-injected knees was noted only for acute scores (P = 0.07).As a proof of concept, BOLD MRI can depict perisynovial changes during progression of experimental arthritis.