Epidemiological features, resistance genes, and clones among community-onset methicillin-resistant Staphylococcus aureus (CO-MRSA) isolates detected in northern Spain
Epidemiological features, resistance genes, and clones among community-onset methicillin-resistant Staphylococcus aureus (CO-MRSA) isolates detected in northern Spain
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DOI：
10.1016/j.ijmm.2012.10.003
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发表时间：
2012-12-01
影响因子：
4.1
通讯作者：
Javier Castillo, Francisco
中科院分区：
文献类型：
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作者：
Gonzalez-Dominguez, Maria;Seral, Cristina;Javier Castillo, Francisco
根据流行病学标准，从本实验室2009-2010年收集的374株MRSA分离株中前瞻性地选择29株社区发病耐甲氧西林金黄色葡萄球菌（CO-MRSA）分离株，以确定西班牙北部社区流行的社区相关性MRSA （CA-MRSA）和卫生保健相关性MRSA （HA-MRSA）克隆。在SCCmec IV型和V型5株（17.2%）、agr I组（ST8和ST2050）、agr II组（ST121）和agr III组（ST30和ST852）中检测到PVL基因。这些菌株均分离自泌尿道等不同临床表现的患者。感染、脓肿或肺炎，多数为急诊患者，隔离前一年无全科医生就诊史。我们认为CA-MRSA在社区发病分离株中的流行率很低（17.2%）。CO-MRSA菌株中ST125-MRSA-IVc （CC5）所占比例较高（58.6%），是西班牙HA-MRSA菌株感染的主要原因。正如我们在本研究中可以证明的那样，这种地方性克隆也在西班牙北部社区传播。在spa型t067分离株中发现了与ant（4’）-Ia和msr(A)存在相关的抗微生物药物耐药性。本研究中大多数CO-MRSA分离株对应于与医院环境更相关的spa类型，这表明MRSA在社区和医院生态位之间存在遗传谱系的交换。(C) 2012 Elsevier GmbH版权所有。
Twenty-nine community-onset methicillin-resistant Staphylococcus aureus (CO-MRSA) isolates were prospectively selected according to epidemiological criteria among 374 MRSA isolates collected in our laboratory during 2009-2010 in order to determine which community-associated MRSA (CA-MRSA) and healthcare-associated MRSA (HA-MRSA) clones are circulating in the community in northern Spain. PVL genes were detected in 5 strains (17.2%) that belonged to SCCmec type IV or V and to the agr group I (ST8 and ST2050), agr group II (ST121), and agr group III (ST30 and ST852). These strains were isolated from patients with different clinical manifestations such as urinary tract. infection, abscess, or pneumonia, and most of them belonged to emergency department patients with no history of visits to General Practitioners (GPs) in the year before the isolation. We considered that the prevalence of CA-MRSA in community-onset isolates was low (17.2%). A high proportion of the CO-MRSA strains (58.6%) were ST125-MRSA-IVc (CC5), responsible for most of the infections caused by HA-MRSA strains in Spain. This endemic clone is also circulating in the community of northern Spain as we could demonstrate in this study. Antimicrobial resistance was found in spa type t067 isolates linked to the presence of ant(4')-Ia and msr(A). Most of the CO-MRSA isolates in this study corresponded to spa types more associated to the hospital environment, suggesting the interchange of genetic lineages of MRSA among community and hospital niches. (C) 2012 Elsevier GmbH. All rights reserved.