Nucleotide sequence analysis of pRS1, a cryptic plasmid from Oenococcus oeni
Nucleotide sequence analysis of pRS1, a cryptic plasmid from Oenococcus oeni
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DOI：
10.1006/plas.1998.1382
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发表时间：
1999-03-01
期刊：
影响因子：
2.6
通讯作者：
Mesas, JM
中科院分区：
文献类型：
--
作者：
Alegre, MT;Rodríguez, MC;Mesas, JM
报道了从西班牙葡萄酒中分离的一株酒酒球菌菌株中发现的一种新的隐蔽质粒pRS 1。核苷酸序列分析（2523 bp）显示存在三个主要的开放阅读框架（ORF），其核苷酸序列和编码蛋白与先前描述的O. oeni。与O. Oeni（即，pLo 13和pOg 32）。它们在同一条链上具有三个主要ORF;这些ORF中的两个推定编码的蛋白质分别与pT 181质粒家族和相关革兰氏阳性细菌质粒的复制（Rep）和重组（Pre）蛋白质表现出同源性;这些质粒含有通过滚环机制进行质粒复制和重组所需的DNA序列（即，双链起源;单链起源;重组特异性位点，RSA和RSB）;最后，所有这些质粒都具有未知功能的第三个ORF。这些特征表明，DRS 1可能与pLo 13和pOg 32共同构成O. oeni。(C)北京：科学出版社.
A new cryptic plasmid, pRS1, from an Oenococcus oeni strain isolated from Spanish wines is reported. Nucleotide sequence analysis (2523 bp) revealed the presence of three major open reading frames (ORFs) whose nucleotide sequence and encoded proteins exhibit high homology with those of pOg32, a previously described plasmid of O. oeni. Common features in other plasmids from O. oeni (i.e., pLo13 and pOg32) have been found in pRS1. They have three major ORFs in the same strand; the putative encoded proteins by two of these ORFs exhibit homology with the replication (Rep) and the recombination (Pre) proteins, respectively, of the pT181 plasmid family and related gram-positive bacteria plasmids; these plasmids contain the DNA sequences required for plasmid replication by the rolling circle mechanism and for recombination (i.e., double-strand origin, DSO; single-strand origin, SSO; recombination-specific sites, RSA and RSB); and finally, all these plasmids have a third ORF of unknown function. These features suggest that DRS1 could constitute together with pLo13 and pOg32 a family of small cryptic plasmids of O. oeni. (C) 1999 Academic Press.