Engineering of polarized tubular structures in a microfluidic device to study calcium phosphate stone formation.
Engineering of polarized tubular structures in a microfluidic device to study calcium phosphate stone formation.
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微流体装置中极化管状结构的工程研究磷酸钙结石的形成。
DOI：
10.1039/c2lc40801e
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发表时间：
2012
期刊：
影响因子：
6.1
通讯作者：
Bandyopadhyay,BidhanC
中科院分区：
文献类型：
--
作者：
Wei,Zengjiang;Amponsah,PrinceK;Al-Shatti,Mariyam;Nie,Zhihong;Bandyopadhyay,BidhanC
该通信描述了通过在微流体（MF）装置中生长上皮细胞来形成具有圆形横截面的管状结构。在这里，我们第一次表明，有可能形成一个单层的极化细胞，包埋在MF设备，可以作为一个在体内的上皮细胞。我们显示：i）感兴趣的特定蛋白质的过表达（即，离子通道和转运蛋白）在MF中的管状结构内是可行的; ii）细胞中Ca 2+的功能动力学信息可以通过在共聚焦显微镜下使用细胞可渗透的Ca 2+探针的显微荧光测定法来测量;以及iii）磷酸钙结石可以在具有Ca 2+转运上皮的MF中在真实的时间内产生。这些数据表明，该MF平台内的管状结构可用作合适的模型，以了解上皮或其他类似细胞微环境中磷酸钙结石形成的分子和药理学基础。
This communication describes the formation of tubular structures with a circular cross-section by growing epithelial cells in a microfluidic (MF) device. Here we show for the first time that it is possible to form a monolayer of polarized cells, embedded within the MF device which can function as an in vivo epithelia. We showed: i) the overexpression of specific protein(s) of interest (i.e., ion channel and transport proteins) is feasible inside tubular structures in MFs; ii) the functional kinetic information of Ca2+ in cells can be measured by microflurometry using cell permeable Ca2+ probe under confocal microscope; and iii) calcium phosphate stones can be produced in real time in MFs with Ca2+ transporting epithelia. These data suggest that tubular structures inside this MF platform can be used as a suitable model to understand the molecular and pharmacological basis of calcium phosphate stone formation in the epithelial or other similar cellular micro environments.