Measurement of OH, O, and NO densities and their correlations with mouse melanoma cell death rate treated by a nanosecond pulsed streamer discharge
Measurement of OH, O, and NO densities and their correlations with mouse melanoma cell death rate treated by a nanosecond pulsed streamer discharge
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DOI：
10.1088/0022-3727/48/42/424006
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
I. Yagi;Y. Shirakawa;Kenta Hirakata;T. Akiyama;S. Yonemori;K. Mizuno;R. Ono;T. Oda
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文献类型：
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作者：
I. Yagi;Y. Shirakawa;Kenta Hirakata;T. Akiyama;S. Yonemori;K. Mizuno;R. Ono;T. Oda
使用纳秒脉冲流光放电等离子体处理培养基中的小鼠黑色素瘤细胞，并测量细胞死亡率与等离子体中活性物质（OH、O 和 NO）密度之间的相关性。用来自玻璃管的 O2/N2 混合物的垂直气流以不同的气体流速和 O2 浓度将等离子体照射到培养基表面。使用激光诱导荧光，在非常接近培养基表面的位置测量活性物质的密度，其中活性物质与培养基相互作用。在 N2 排放 (O2 = 0%) 的情况下，气体流量的增加会降低 OH 密度，因为它通过稀释水蒸气来降低水蒸气浓度，而水蒸气是 OH 生成所需的。气体流速的增加也导致细胞死亡率降低。另一方面，在 O2/N2 放电的情况下，固定流速下 O2 浓度的增加不会影响细胞死亡率，尽管它会显着改变 O 和 NO 密度。这些发现表明，一些源自水蒸气的活性物质（例如 OH）是导致黑色素瘤细胞死亡的原因，而来自 O2 的活性物质（例如 O 和 NO）则不太可能造成黑色素瘤细胞死亡。它们还表明了细胞处理中培养基表面水分蒸发的重要性。
Mouse melanoma cells in a culture medium are treated using a nanosecond pulsed streamer discharge plasma and the correlations between the rate of cell death and the densities of reactive species (OH, O, and NO) in the plasma are measured. The plasma is irradiated onto the culture medium surface with a vertical gas flow of an O2/N2 mixture from a glass tube at various gas flow rates and O2 concentrations. The densities of the reactive species are measured very close to the culture medium surface, where the reactive species interact with the culture medium, using laser-induced fluorescence. In the case of the N2 discharge (O2 = 0%), an increase in gas flow rate decreases OH density because it lowers the water vapor concentration by diluting the vapor, which is required for OH production. The increase in gas flow rate also leads to a decreased cell death rate. In the case of the O2/N2 discharge, on the other hand, an increase in O2 concentration at a fixed flow rate does not affect the rate of cell death, although it considerably changes the O and NO densities. These findings indicate that some reactive species derived from water vapor such as OH are responsible for the melanoma cell death, whereas those from O2, such as O and NO, are less likely responsible. They also indicate the importance of water evaporation from the culture medium surface in cell treatment.