First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis
First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis
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DOI：
10.1002/elps.1150180820
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发表时间：
1997-08-01
期刊：
影响因子：
2.9
通讯作者：
Hecker, M
中科院分区：
文献类型：
--
作者：
Antelmann, H;Bernhardt, J;Hecker, M
总结了枯草芽孢杆菌蛋白质在二维凝胶上的鉴定及其调控的数据，包括56个蛋白质点的鉴定。分析了枯草芽孢杆菌在指数生长过程中、在葡萄糖或磷酸盐饥饿过程中、或在施加应力（如热激、盐和乙醇应力以及氧化应力）之后合成的蛋白质的模式。蛋白质点的N-末端测序允许在2-D凝胶上鉴定93种蛋白质，这些蛋白质是合成氨基酸和核苷酸、生成ATP、糖酵解、磷酸戊糖循环、柠檬酸循环以及适应各种应激条件所需的。计算机辅助分析的2-D凝胶被用来监测超过130个蛋白质点的合成概况。在指数生长过程中执行管家功能的蛋白质在应激和饥饿过程中显示出降低的合成速率，而在应激和饥饿过程中诱导的斑点被归类为由单一刺激或一组相关刺激诱导的特异性应激蛋白，或由各种完全不同的刺激诱导的一般应激蛋白。为了建立B的响应调控图谱，启动了全局调节子中突变体的分析。枯草杆菌。这些研究表明，替代西格玛因子西格玛（B）参与几乎所有的一般应力蛋白的调节，phoPR双组分系统是诱导大部分，但不是所有的蛋白诱导磷酸盐饥饿所需的。
Data on the identification of proteins of Bacillus subtilis on two-dimensional (2-D) gels as well as their regulation are summarized and the identification of 56 protein spots is included. The pattern of proteins synthesized in Bacillus subtilis during exponential growth, during starvation for glucose or phosphate, or after the imposition of stresses like heat shock, salt-and ethanol stress as well as oxidative stress was analyzed. N-terminal sequencing of protein spots allowed the identification of 93 proteins on 2-D gels, which are required for the synthesis of amino acids and nucleotides, the generation of ATP, for glycolyses, the pentose phosphate cycle, the citric acid cycle as well as for adaptation to a variety of stress conditions. A computer-aided analysis of the 2-D gels was used to monitor the synthesis profile of more than 130 protein spots. Proteins performing housekeeping functions during exponential growth displayed a reduced synthesis rate during stress and starvation, whereas spots induced during stress and starvation were classified as specific stress proteins induced by a single stimulus or a group of related stimuli, or as general stress proteins induced by a variety of entirely different stimuli. The analysis of mutants in global regulators was initiated in order to establish a response regulation map for B. subtilis. These investigations demonstrated that the alternative sigma factor sigma(B) is involved in the regulation of almost all of the general stress proteins and that the phoPR two-component system is required for the induction of a large part but not all of the proteins induced by phosphate starvation.