MtDNA Analysis for Genetic Identification of Forensically Important Sarcophagid Flies (Diptera: Sarcophagidae) in China
MtDNA Analysis for Genetic Identification of Forensically Important Sarcophagid Flies (Diptera: Sarcophagidae) in China
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DOI：
10.1093/jme/tjv131
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发表时间：
2015-11
期刊：
影响因子：
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通讯作者：
Changquan Zhang;Xiaoliang Fu;K. Xie;Weitao Yan;Yadong Guo
中科院分区：
文献类型：
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作者：
Changquan Zhang;Xiaoliang Fu;K. Xie;Weitao Yan;Yadong Guo
摘要基于DNA的技术已经越来越多地应用于法医学上重要的石螨物种鉴定，因为它们通常在形态上并不明显，特别是对于未成熟的标本。线粒体基因组已被广泛用于物种水平的鉴定。虽然中国麻蝇的短片段（200-600 bp）序列已在GenBank中保存，但其条形码区和完整的细胞色素氧化酶亚基I（COI）和COII序列仍不可用。本研究从中国18个省、市、自治区、直辖市的29个地点采集了78只石蝇标本，分别代表中国石蝇科17种。首次获得了中国肉蝇类重要分类群的线粒体COI和COII序列数据，为中国种类鉴定提供了参考。系统发育分析表明，COI和COII序列是有用的，以确定大多数麻蝇物种。本研究结果将有助于中国麻蝇科在法医昆虫学中的应用。然而，线粒体DNA作为物种鉴定的应用需要非常谨慎，并应研究额外的标记和方法，以确保未来鉴定的准确性。
ABSTRACT DNA-based technologies have been increasingly used in species determination of forensically important sarcophagids, as they are often not morphologically distinct, especially for the immature specimens. The mitochondrial genome has been broadly used for species-level identifications. Although Chinese sarcophagid sequences of short fragments (200–600 bp) had been deposited in GenBank, the barcode region and the complete cytochrome oxidase subunit I (COI) and COII sequences are still unavailable. In this study, 78 sarcophagid fly specimens, representing 17 Chinese sarcophagid species, were collected from 29 locations in 18 Chinese provinces. Sequence data of the mitochondrial COI and COII of the most important Chinese flesh fly taxa associated with cadavers were presented for first time, which serve as reference standards for Chinese species determination. Phylogenetic analysis showed that the COI and COII sequences were useful for identifying most sarcophagid species. The results of this research will be conductive for implementation of the Chinese Sarcophagidae in forensic entomology. However, the application of mitochondrial DNA as species identifier requires great circumspection and additional markers and methods should be studied to ensure accuracy of identification in the future.