Highly effective volatile organic compound dissolving strategy based on mist atomization for odorant biosensors
Highly effective volatile organic compound dissolving strategy based on mist atomization for odorant biosensors
复制标题
基于雾化的气味生物传感器高效挥发性有机化合物溶解策略
DOI：
10.1016/j.aca.2020.09.043
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发表时间：
2020
影响因子：
6.2
通讯作者：
Kanzaki Ryohei
中科院分区：
文献类型：
--
作者：
Terutsuki Daigo;Mitsuno Hidefumi;Sato Kohei;Sakurai Takeshi;Mase Nobuyuki;Kanzaki Ryohei
挥发性有机化合物(VOC)混合物的检测在医疗和安全领域具有重要意义。基于受体的气味生物传感器可以灵敏和选择性地检测溶液中的气味分子；然而，气味分子通常以VOCs的形式存在于空气中，并且表现出较差的水溶解性。因此，能够使用便携式系统溶解难溶于水的VOCs的技术对于实际生物传感器的应用是必不可少的。我们以前提出了一种基于水雾化的VOC溶解方法，通过产生细小的气泡来增加比表面积，作为概念验证；然而，该系统是基于实验室的(非流动的)，并且溶解被限制为一个VOC。在本研究中，我们建立了一种高效的基于雾化的VOC溶解方法，该方法可以在现场使用。这种新方法无需使用任何有机溶剂，在蒸馏水(DW)中可在1min内快速溶解具有不同官能团的稀溶VOC混合物。钙成像显示，气味受体13a表达的Sf21细胞(或13a细胞)对混合物中的1-辛烯-3-醇有反应。此外，我们成功地开发了一种现场可展开的原型真空和溶解系统，该系统具有简单的配置，可以有效地捕获并快速溶解空气中的1-辛烯-3-醇。这项研究提出了一种现场可部署的系统，适合于溶解各种空气中的气味分子，因此是一种实用的策略，用于气味生物传感器。
The detection of volatile organic compound (VOC) mixtures is crucial in the medical and security fields. Receptor-based odorant biosensors sensitively and selectively detect odorant molecules in a solution; however, odorant molecules generally exist as VOCs in the air and exhibit poor water solubility. Therefore, techniques that enable the dissolution of poorly water-soluble VOCs using portable systems are essential for practical biosensors’ applications. We previously proposed a VOC dissolution method based on water atomization to increase the surface area via the generation of fine bubbles, as a proof-of-concept; however, the system was lab-based (non-mobile) and the dissolution was limited to one VOC. In this study, we established a highly effective VOC dissolution method based on mist atomization that can be used in the field. This new method demonstrated a rapid dissolution potential of a sparsely-soluble VOC mixture with various functional groups in distilled water (DW) within 1 min, without the use of any organic solvents. Calcium imaging revealed that odorant receptor 13a-expressing Sf21 cells (Or13a cells) responded to 1-octen-3-ol in the mixture. Further, we successfully developed a field-deployable prototype vacuum and dissolution system with a simple configuration that efficiently captured and rapidly dissolved airborne 1-octen-3-ol in DW. This study proposes a field-deployable system that is appropriate for solubilizing various airborne odorant molecules and therefore is a practical strategy to use in the context of odorant biosensors.