ENVIRONMENTAL EXPOSURE TO COCKROACH ALLERGENS - ANALYSIS WITH MONOCLONAL ANTIBODY-BASED ENZYME IMMUNOASSAYS
ENVIRONMENTAL EXPOSURE TO COCKROACH ALLERGENS - ANALYSIS WITH MONOCLONAL ANTIBODY-BASED ENZYME IMMUNOASSAYS
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DOI：
10.1016/0091-6749(91)90009-d
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发表时间：
1991-02-01
影响因子：
14.2
通讯作者：
CHAPMAN, MD
中科院分区：
文献类型：
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作者：
POLLART, SM;SMITH, TF;CHAPMAN, MD
定量双位点单克隆抗体（MAb）为基础的酶联免疫测定两种蟑螂（CR）过敏原，Bla g I和Bla g II，已被开发和用于测量室内灰尘样品中的过敏原水平。从弗吉尼亚州夏洛茨维尔的两名哮喘患者的CR感染家中收集的灰尘，显示Bla g I水平的广泛变化，取决于灰尘收集的位置。来自厨房地板和橱柜的灰尘含有50倍以上的过敏原（平均值为10，755 U/gm灰尘），而来自卧室和软垫家具的灰尘（平均值为204 U/gm）。在佐治亚州亚特兰大市的22名哮喘儿童和16名无哮喘的对照组的卧室和起居室收集了145份灰尘样本。38个家庭中有27个（17/22名哮喘儿童; 10/16名对照受试者）可检测到Bla g I（4至1340 U/gm灰尘）。Bla g II水平在来自特拉华州威尔明顿的40个厨房、卧室和起居室样品中进行了测定。在厨房地板灰尘中检测到最高水平的Bla g II（300 U/gm灰尘）。此外，大约20%的没有可见的CR感染证据的家庭在至少一个灰尘样品中具有显著水平的Bla g II（> 4 U/gm灰尘）。我们的研究结果表明，CR可能是一个隐藏的过敏原在家里。厨房似乎是CR-过敏原积累的主要场所，但在家中的其他场所也可以发现显著的CR-过敏原水平。基于MAb的测定可用于定量CR过敏原的环境暴露。单克隆抗体在流行病学研究中应该是非常有用的，以建立CR-过敏原暴露水平，应被视为过敏性疾病，特别是哮喘的发展的危险因素。
Quantitative two-site monoclonal antibody (MAb)-based enzyme-linked immunoassays for two cockroach (CR) allergens, Bla g I and Bla g II, have been developed and used to measure allergen levels in house-dust samples. Dust collected from the CR-infested homes of two patients with asthma from Charlottesville, Va., demonstrated wide variation in the levels of Bla g I, depending on the location of dust collection. Dust from kitchen floors and cabinets contained 50-fold more allergen (mean, 10,755 U/gm of dust) than dust from bedrooms and upholstered furniture (mean, 204 U/gm). One hundred forty-five dust samples were collected from the bedrooms and living rooms of 22 children with asthma and 16 control subjects without asthma living in Atlanta, Ga. Twenty-seven of the 38 homes (17/22 children with asthma; 10/16 control subjects) had detectable Bla g I (4 to 1340 U/gm of dust). Bla g II levels were assayed in 40 kitchen, bedroom, and living room samples from homes in Wilmington, Del. Highest levels of Bla g II were detected in kitchen-floor dust (300 U/gm of dust). Additionally, approximately 20% of homes with no visual evidence of CR infestation had significant levels of Bla g II in at least one dust sample (> 4 U/gm of dust). Our results demonstrate that CR may be an occult allergen in homes. The kitchen appears to be the primary site of CR-allergen accumulation, but significant CR-allergen levels can also be found at other sites in the home. The MAb-based assays can be used for quantitation of environmental exposure to CR allergens. MAbs should be very useful in epidemiologic studies to establish levels of CR-allergen exposure that should be considered as a risk factor for the development of allergic diseases, particularly asthma.