Uterotropic actions of relaxin in prepubertal gilts.
Uterotropic actions of relaxin in prepubertal gilts.
复制标题
青春期前后备母猪松弛素的向子宫作用。
DOI：
10.1095/biolreprod42.5.769
复制
发表时间：
1990
影响因子：
3.6
通讯作者：
R. Anthony
中科院分区：
文献类型：
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作者：
J. A. Hall;T. Cantley;B. N. Day;R. Anthony
松弛素（RLX）的子宫收缩作用已在大鼠和小鼠中得到证实，但未在其他物种中得到证实。进行实验以评估（1）RLX是否在青春期前后备母猪中具有促子宫作用和（2）RLX诱导的子宫组织吸水是否由组胺介导。接受的青春期前后备母猪（i.m.）每6小时给予0.5 mg pRLX（大于或等于3000 U/mg; n = 10）的0.1 M磷酸盐缓冲盐水（PBS）溶液或PBS载体（n = 8），持续54小时。此外，每组的一半接受（1）组胺受体拮抗剂（300 mg i.m.的每种）、马来酸吡拉明（H1）和西咪替丁（H2），分别在PBS和玉米油中，或（2）对照媒介物，从开始RLX处理前12小时开始，每6小时持续66小时。RLX给药刺激青春期前后备母猪的子宫重量增加2倍（p ≤ 0.05）。RLX给药后备母猪的子宫含水量和干重增加（p ≤ 0.05），表明子宫总重量增加。与组胺受体拮抗剂的共同治疗没有改变RLX诱导的子宫含水量或干重的变化。与对照组相比，RLX处理后备母猪的可溶性蛋白和DNA的子宫含量更高（p ≤ 0.05）。RLX治疗倾向于（p约0.08）降低子宫组织的蛋白质和DNA比率，并增加（p小于或等于0.05）子宫DNA占子宫干重的百分比。（250字处删节）
Uterotropic actions of relaxin (RLX) have been demonstrated in rats and mice but not in other species. Experiments were conducted to assess (1) if RLX has uterotropic effects in prepubertal gilts and (2) if RLX-induced imbibition of water by uterine tissues is mediated by histamine. Prepubertal gilts received (i.m.) either 0.5 mg pRLX (greater than or equal to 3000 U/mg; n = 10) in 0.1 M phosphate-buffered saline (PBS) or PBS vehicle (n = 8) every 6 h for 54 h. Additionally, one-half of each group received either (1) the histamine receptor antagonists (300 mg i.m. of each), pyrilamine maleate (H1) and cimetidine (H2), in PBS and corn oil, respectively, or (2) control vehicles every 6 h for 66 h beginning 12 h prior to initiation of RLX treatment. Treatment with RLX stimulated a twofold increase (p less than or equal to 0.05) in uterine weight in prepubertal gilts. Increases in total uterine weight were manifested by greater (p less than or equal to 0.05) uterine water content and dry weight in RLX-treated gilts. Cotreatment with histamine receptor antagonists did not alter RLX-induced changes in uterine water content or dry weight. Uterine contents of soluble protein and DNA were greater (p less than or equal to 0.05) in RLX-treated gilts compared to controls. Treatment with RLX tended (p approximately 0.08) to reduce protein; DNA ratio of uterine tissues and increased (p less than or equal to 0.05) uterine DNA as a percentage of uterine dry weight.(ABSTRACT TRUNCATED AT 250 WORDS)
影响因子：
--
作者：
LINGHAM, RB;STANCEL, GM;LOOSEMITCHELL, DS
通讯作者：
LOOSEMITCHELL, DS