Synthetic biology of cyanobacteria: unique challenges and opportunities.
Synthetic biology of cyanobacteria: unique challenges and opportunities.
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DOI：
10.3389/fmicb.2013.00246
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发表时间：
2013
影响因子：
5.2
通讯作者：
Pakrasi HB
中科院分区：
文献类型：
--
作者：
Berla BM;Saha R;Immethun CM;Maranas CD;Moon TS;Pakrasi HB
光合生物，尤其是蓝细菌，作为可再生燃料、散装和特种化学品以及营养产品的来源具有广阔的前景。合成生物学工具可以帮助释放蓝细菌的这些功能潜力，但不幸的是，这些生物体的工具开发落后于酿酒酵母和大肠杆菌。虽然在许多情况下，这些生物体可能比某些异养生物更难以作为合成生物学的“基础”菌株，但自养生物在生物技术应用中的独特优势以及提高对光合作用的理解的科学重要性保证了将这些系统发展成类似于“绿色大肠杆菌”的东西。在这篇综述中，我们强调了蓝藻合成生物学方法发展的独特挑战和机遇。我们回顾了在各种蓝藻菌株中构建靶向突变体的经典方法和最近开发的方法，并就哪些遗传工具可以最大程度地扩展在此类菌株中设计新功能的能力提供了观点。同样，我们回顾了蓝藻合成生物学的发展最需要哪些遗传部分。最后，我们重点介绍了构建蓝藻代谢的基因组规模模型并使用这些模型来测量自养代谢特性的最新方法。在本文中，我们讨论了昼夜自养生活方式的一些独特挑战，以及蓝藻合成生物学和生物技术的发展必须如何适应这些限制。
Photosynthetic organisms, and especially cyanobacteria, hold great promise as sources of renewably-produced fuels, bulk and specialty chemicals, and nutritional products. Synthetic biology tools can help unlock cyanobacteria's potential for these functions, but unfortunately tool development for these organisms has lagged behind that for S. cerevisiae and E. coli. While these organisms may in many cases be more difficult to work with as “chassis” strains for synthetic biology than certain heterotrophs, the unique advantages of autotrophs in biotechnology applications as well as the scientific importance of improved understanding of photosynthesis warrant the development of these systems into something akin to a “green E. coli.” In this review, we highlight unique challenges and opportunities for development of synthetic biology approaches in cyanobacteria. We review classical and recently developed methods for constructing targeted mutants in various cyanobacterial strains, and offer perspective on what genetic tools might most greatly expand the ability to engineer new functions in such strains. Similarly, we review what genetic parts are most needed for the development of cyanobacterial synthetic biology. Finally, we highlight recent methods to construct genome-scale models of cyanobacterial metabolism and to use those models to measure properties of autotrophic metabolism. Throughout this paper, we discuss some of the unique challenges of a diurnal, autotrophic lifestyle along with how the development of synthetic biology and biotechnology in cyanobacteria must fit within those constraints.
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影响因子：
2.9
作者：
Cheah, Yi Ern;Albers, Stevan C.;Peebles, Christie A. M.
通讯作者：
Peebles, Christie A. M.
DOI：
10.1073/pnas.0600927103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Dühring, U;Axmann, IM;Wilde, A
通讯作者：
Wilde, A
DOI：
10.1007/978-1-61779-065-2_11
发表时间：
2011-01-01
期刊：
CDNA LIBRARIES: METHODS AND APPLICATIONS
影响因子：
--
作者：
Engler, Carola;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
4.4
作者：
Chaurasia, Akhilesh Kumar;Apte, Shree Kumar
通讯作者：
Apte, Shree Kumar