S-Glycosylation of Fluensulfone in Tomatoes: An Important Way of Fluensulfone Metabolism
S-Glycosylation of Fluensulfone in Tomatoes: An Important Way of Fluensulfone Metabolism
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番茄中氟苯磺隆的S-糖基化：氟苯磺隆代谢的重要途径
DOI：
10.1021/acs.jafc.1c04725
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发表时间：
2021
影响因子：
6.1
通讯作者：
Nanshan Du
中科院分区：
文献类型：
--
作者：
Jing Jing Zhan;Wanxue Cao;Pengxuan Xi;Li Li;Songtao Qiao;Huawei Luo;Jianye Zhang;Xiangyang Liu;Nanshan Du
氟砜（FSF）由于其非熏蒸施用方法而越来越受欢迎。然而，目前对FSF在植物体内的代谢机制研究较少。本研究以番茄幼苗为材料，通过水培试验，探讨番茄体内FSF代谢、番茄内源苷类物质的调节以及番茄细胞内过氧化氢的清除之间的关系。FSF仅在番茄下部茎中积累; FSF主要在根部代谢为S-糖基化结合物，且根部是代谢产物含量最高的组织;上部叶中未检测到FSF及其代谢产物。2 mg/L的FSF胁迫7 d后，番茄幼苗茎中糖和苷类物质含量显著增加。FSF对葡萄糖代谢途径中某些化合物的含量有影响。FSF胁迫下，硫代葡萄糖苷合成途径中的3种前体化合物（高甲硫氨酸、异亮氨酸和l-酪氨酸）含量显著增加，表明FSF可能与它们竞争UGT 74 B1。此外，FSF诱导的黄酮苷可能在清除过氧化氢的过程中发挥作用。这一研究为许多含磺酰基的异生物素在植物中的命运提供了启示。
Fluensulfone (FSF) becomes increasingly popular because of its nonfumigation application method. However, studies on the metabolic mechanism of FSF in plants are lacking. Here, tomato seedling was cultivated in hydroponic media to investigate the connection among FSF’s metabolism in tomato, the regulation of tomato endogenous glycosides, and the elimination of hydrogen peroxide in tomato cells. The accumulation of FSF was only detected in the lower stems of tomatoes; FSF was mainly metabolized intoS-glycosylated conjugates in the roots, and the roots were the tissues with the highest metabolite content; and no FSF and metabolites were detected in the upper leaves. In response to FSF stress (2 mg/L for 7 d), the content of sugar and glycosides in the stems of tomato seedlings significantly increased. The amount of some compounds on the pathway related to glucose was affected by FSF. The three precursor compounds (homomethioine, isoleucine, andl-tyrosine) in the pathway of glucosinolate biosynthesis increased significantly under the stress of FSF, which indicates that FSF may compete with them for UGT74B1. Besides, FSF-induced flavonoid glycosides may play a role in the process of removing hydrogen peroxide. This research provides inspiration for the fate of many xenobiotics containing sulfonyl groups in plants.