Characterization of 3-Oxacyl-Acyl Carrier Protein Reductase Homolog Genes in Pseudomonas aeruginosa PAO1
Characterization of 3-Oxacyl-Acyl Carrier Protein Reductase Homolog Genes in Pseudomonas aeruginosa PAO1
复制标题
铜绿假单胞菌 PAO1 中 3-草酰基-酰基载体蛋白还原酶同源基因的表征
DOI：
10.3389/fmicb.2019.01028
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发表时间：
2019-05-22
影响因子：
5.2
通讯作者：
Wang, Hai-Hong
中科院分区：
文献类型：
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作者：
Guo, Qiao-Qiao;Zhang, Wen-Bin;Wang, Hai-Hong
细菌3-氧代酰基-ACP还原酶（OAR）催化脂肪酸合成途径中的3-氧代酰基-ACP还原步骤。铜绿假单胞菌基因组中至少12个基因被注释为OAR编码基因。在这项研究中，我们的特点，这些基因的功能与生化和遗传技术。除了编码脂肪酸合成中必需蛋白FabG的PA 2967之外，只有PA 4389和PA 4786基因产物具有OAR活性，并且这两个基因的单一缺失降低了铜绿假单胞菌产生几种特异性群体感应（QS）信号的能力。然而，PA 4389和PA 4786在脂肪酸合成中没有关键作用。此外，虽然大多数OAR同源物没有OAR活性，但有些可能在碳利用中起作用。PA 3128产物可能在TCA循环中发挥作用，PA 0182和PA 1470似乎是利用几种氨基酸所必需的。其余的OAR同源物在碳利用中没有作用，但是这些基因中的一个的缺失可能会影响铜绿假单胞菌毒力因子的产生。我们的结论是，大多数OAR同源基因不编码OAR酶，这些蛋白质不功能的脂肪酸合成。重要性我们报道，虽然所有铜绿假单胞菌OAR同系物具有相似的结构和保守的催化三联体的细菌OAR酶，只有少数OAR同系物具有OAR活性。
Bacterial 3-oxoacyl-ACP reductase (OAR) catalyzes the 3-oxoacyl-ACP reduction step in the fatty acid synthesis pathway. At least 12 genes in the Pseudomonas aeruginosa genome are annotated as OAR-encoding genes. In this study, we characterized the functions of these genes with biochemical and genetic techniques. With the exception of PA2967, which encodes FabG, an essential protein in fatty acid synthesis, only the PA4389 and PA4786 gene products had OAR activity, and the single deletion of these two genes reduced the ability of P. aeruginosa to produce several specific quorum-sensing (QS) signals. However, PA4389 and PA4786 do not have key roles in fatty acid synthesis. Moreover, although most OAR homologs had no OAR activity, some may function in carbon utilization. The PA3128 product may play a role in the TCA cycle, and PA0182 and PA1470 seem to be required for the utilization of several amino acids. The rest of the OAR homologs have no roles in carbon utilization, but the deletion of one of these genes might affect the production of virulence factors by P. aeruginosa. We conclude that most OAR homolog genes do not encode OAR enzymes, and that these proteins do not function in fatty acid synthesis. Importance We report that although all P. aeruginosa OAR homologs have similar structures and the conserved catalytic triad of the bacterial OAR enzymes, only a few OAR homologs have OAR activity.