A hepatopancreas-specific C-type lectin from the Chinese shrimp Fenneropenaeus chinensis exhibits antimicrobial activity
A hepatopancreas-specific C-type lectin from the Chinese shrimp Fenneropenaeus chinensis exhibits antimicrobial activity
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DOI：
10.1016/j.molimm.2007.06.355
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发表时间：
2008-01-01
影响因子：
3.6
通讯作者：
Wang, Jin-Xing
中科院分区：
文献类型：
--
作者：
Sun, Yun-Dong;Fu, Li-Dong;Wang, Jin-Xing
凝集素作为模式识别受体、调理素或效应分子在动物先天免疫应答中发挥重要作用。在这里，我们报道了一种来自中国明对虾肝胰腺的新型肝胰腺特异性C型凝集素，命名为Fc-hsL。该基因全长571 bp，开放阅读框为480 bp，编码159个氨基酸残基。Fc-hsL含有一个信号肽和一个C型凝集素样结构域（CTLD）或碳水化合物识别结构域（CRD）。它具有EPN（Glu-Pro-Asn）基序，具有预测的甘露糖特异性配体结合位点。Fc-hsL在正常对虾肝胰腺中组成型表达，经细菌或病毒攻击后表达上调。在其他组织中未检测到Fc-hsL，但在免疫攻击的虾的胃中被诱导。Fc-hsL蛋白在细菌和病毒攻击的对虾的血淋巴和肝胰腺中均被检测到。重组成熟Fc-hsL无血凝活性，但对革兰氏阳性菌和革兰氏阴性菌有钙依赖性凝集活性。rFc-hsL还具有与一些革兰氏阳性和革兰氏阴性细菌的结合活性以及对一些细菌和真菌的高抗微生物活性。这些重组Fc hsL的体外功能是不依赖于钙离子的。Fc-hsL可能作为模式识别受体参与中国对虾的抗菌防御，并可能作为效应子参与中国对虾的天然免疫。(c)2007爱思唯尔有限公司保留所有权利。
Lectins play important roles in animal innate immune responses by serving as pattern recognition receptors, opsonins, or effector molecules. Here, we report a novel hepatopancreas-specific C-type lectin, designated Fc-hsL, from the hepatopancreas of the Chinese shrimp, Fenneropenaeus chinensis. The cDNA of Fc-hsL is 571 bp long with a 480 bp open reading frame that encodes a 159-residue protein. Fc-hsL contains a signal peptide and a single C-type lectin-like domain (CTLD) or carbohydrate recognition domain (CRD). It has an EPN(Glu-Pro-Asn) motif with a predicted ligand-binding site specific for mannose. Fc-hsL was constitutively expressed in the hepatopancreas of normal shrimp, and its expression was up-regulated following challenge of shrimp with bacteria or virus. Fc-hsL was not detected in other tissues but was induced in the stomach of immune-challenged shrimp. Fc-hsL protein was detected in both hemolymph and the hepatopancreas of bacteria- and virus-challenged shrimp. Recombinant mature Fc-hsL has no hemagglutinating activity, but calcium-dependent agglutinating activity against some Gram-positive and Gram-negative bacteria was detected. The rFc-hsL also has binding activity to some Gram-positive and Gram-negative bacteria and high antimicrobial activity against some bacteria and fungi. These in vitro functions of recombinant Fc-hsL were calcium-independent. Fc-hsL may act as a pattern recognition receptor in antibacterial defense and as an effector in innate immunity of Chinese shrimp. (c) 2007 Elsevier Ltd. All rights reserved.