Characterization of lignocellulose particles during lignocellulose solubilization by Clostridium thermocellum
Characterization of lignocellulose particles during lignocellulose solubilization by Clostridium thermocellum
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DOI：
10.1080/09168451.2017.1364619
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发表时间：
2017-01-01
影响因子：
1.6
通讯作者：
Karita, Shuichi
中科院分区：
文献类型：
--
作者：
Ichikawa, Shunsuke;Nishida, Ayami;Karita, Shuichi
热纤梭菌是一种高效的木质纤维素溶解菌，是木质纤维素利用的候选菌。据报道，C. Thermocellum有效地降解纯化的纤维素底物，但不能完全降解研磨的木质纤维素粉末。对一种木质纤维素残渣中纤维素和半纤维素含量进行了测定。thermocellum表明C.热纤维素降解纤维素和半纤维素相同。显微镜观察表明，C.热纤素显著降解小尺寸的木质纤维素颗粒，但其仅部分降解较大尺寸的颗粒。大颗粒的木质素含量高于小颗粒。剩余的大尺寸颗粒包括血管组织。这些结果表明，木质素结构，如维管组织研磨木质纤维素是不太容易受到细菌木质纤维素溶解。
Clostridium thermocellum is a candidate bacterium for lignocellulose utilization due to its efficient lignocellulose solubilization ability. It has been reported that C. thermocellum efficiently degrades purified cellulose substrates, but cannot completely degrade milled lignocellulose powders. Evaluation of cellulose and hemicellulose contents in a lignocellulose residue after the cultivation of C. thermocellum indicated that C. thermocellum degraded cellulose and hemicellulose equally. Microscopic observations demonstrated that C. thermocellum significantly degraded small-sized lignocellulose particles, but it only partially degraded the larger sized particles. The lignin content of the large-sized particles was higher than that of the small particles. The remained large-sized particles included vascular tissues. These results suggest that the lignified structures such as vascular tissues in milled lignocellulose were less susceptible to bacterial lignocellulose solubilization.