Thermal gelation and tissue adhesion of biomimetic hydrogels
Thermal gelation and tissue adhesion of biomimetic hydrogels
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DOI：
10.1088/1748-6041/2/4/001
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发表时间：
2007-12-01
影响因子：
4
通讯作者：
Messersmith, Phillip B.
中科院分区：
文献类型：
--
作者：
Burke, Sean A.;Ritter-Jones, Marsha;Messersmith, Phillip B.
海洋和淡水贻贝是天然和人造表面臭名昭著的污垢，分泌特殊的蛋白质粘合剂，快速和持久地附着在潮湿的底物上。考虑到贻贝黏附蛋白的强大和防水特性，在仿生合成聚合物材料中模仿其黏附特性具有很大的潜力。这些蛋白质的一个重要组成部分是L-3，4-二羟基苯丙氨酸，这是一种被认为通过氧化和交联反应促进贻贝胶固化的氨基酸。含有DOPA残基的合成聚合物以前被证明在引入氧化剂后可以交联成水凝胶。在这里，我们介绍了一种刺激响应凝胶形成贻贝黏附蛋白模拟聚合物的策略。从二棕榈酰磷脂酰胆碱和二肉豆蔻酰磷脂酰胆碱的混合物中设计了具有37℃双层熔化转变的脂囊，并利用该脂囊在从环境温度加热到生理温度时释放隔离的氧化剂。比色法研究表明，高碘酸钠脂质体在相变温度释放其货物，当与DOPA功能化的聚乙二醇聚合物一起使用时，交联型聚合物水凝胶迅速固化。通过脂质体/聚合物水凝胶在猪真皮组织表面的原位热凝胶化，确定了该仿生体系的组织黏附性能。粘接强度测试表明，在相同条件下，粘附性水凝胶形成的粘结强度(平均值=35.1kPa，SD=12.5kPa，n=11)是纤维蛋白胶对照组的数倍。结果表明，这种仿生策略可能用于软组织修复。
Marine and freshwater mussels are notorious foulers of natural and manmade surfaces, secreting specialized protein adhesives for rapid and durable attachment to wet substrates. Given the strong and water-resistant nature of mussel adhesive proteins, significant potential exists for mimicking their adhesive characteristics in bioinspired synthetic polymer materials. An important component of these proteins is L-3,4-dihydroxylphenylalanine ( DOPA), an amino acid believed to contribute to mussel glue solidification through oxidation and crosslinking reactions. Synthetic polymers containing DOPA residues have previously been shown to crosslink into hydrogels upon the introduction of oxidizing reagents. Here we introduce a strategy for stimuli responsive gel formation of mussel adhesive protein mimetic polymers. Lipid vesicles with a bilayer melting transition of 37 degrees C were designed from a mixture of dipalmitoyl and dimyristoyl phosphatidylcholines and exploited for the release of a sequestered oxidizing reagent upon heating from ambient to physiologic temperature. Colorimetric studies indicated that sodium-periodate-loaded liposomes released their cargo at the phase transition temperature, and when used in conjunction with a DOPA-functionalized poly( ethylene glycol) polymer gave rise to rapid solidification of a crosslinked polymer hydrogel. The tissue adhesive properties of this biomimetic system were determined by in situ thermal gelation of liposome/polymer hydrogel between two porcine dermal tissue surfaces. Bond strength measurements showed that the bond formed by the adhesive hydrogel ( mean = 35.1 kPa, SD = 12.5 kPa, n = 11) was several times stronger than a fibrin glue control tested under the same conditions. The results suggest a possible use of this biomimetic strategy for repair of soft tissues.