Defects in contact-stimulated gliding during aggregation by Myxococcus xanthus.
Defects in contact-stimulated gliding during aggregation by Myxococcus xanthus.
复制标题
黄色粘球菌聚集过程中接触刺激滑动的缺陷。
DOI：
10.1128/jb.172.11.6476-6493.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Zissler,JF
中科院分区：
文献类型：
--
作者：
Kalos,M;Zissler,JF
在发育过程中，黄色粘球菌细胞向聚集灶滑动并产生紧凑的多细胞土丘。我们研究了接触刺激滑动中有缺陷的应变的发展。接触刺激涉及受相邻细胞之间的接触影响的机制，其刺激单细胞的滑动运动性（Hodgkin和Kaiser，Proc. Natl. Acad. Sci. USA 74：2938-2942，1977; Hodgkin和Kaiser，Mol. Genet将军171：167-176，1979）。大多数突变体在影响接触刺激的单个基因（cgl基因）中含有突变，能够在发育过程中形成聚集灶。然而，聚集体是弥漫性的，这表明接触刺激是重要的形态发生的运动过程中聚集。在cglF 3基因中含有突变的突变体显示出聚集的显著延迟，表明cglF 3基因影响刺激细胞移动到病灶的机制或影响协调早期细胞行为的机制。含有cglF 3突变与cglB、cglC、cglE或cglF 1突变组合的突变体具有严重的聚集缺陷，并且未能从早期延迟中恢复。含有两个cgl突变的突变体缺陷的严重性表明cgl基因对发育至关重要。我们提出cgl基因刺激细胞运动或控制聚集过程中细胞之间的特定接触。
During development, Myxococcus xanthus cells glide toward foci of aggregation and produce compact multicellular mounds. We studied development in strains with defects in contact-stimulated gliding. Contact stimulation involves a mechanism influenced by contacts between neighboring cells which stimulates the gliding motility of single cells (Hodgkin and Kaiser, Proc. Natl. Acad. Sci. USA 74:2938-2942, 1977; Hodgkin and Kaiser, Mol. Gen. Genet. 171:167-176, 1979). Most mutants containing a mutation in a single gene affecting contact stimulation (cgl gene) were able to form foci of aggregation during development. However, the aggregates were diffuse, suggesting that contact stimulation is important for morphogenetic movements during aggregation. A mutant containing a mutation in the cglF3 gene showed a striking delay in aggregation, suggesting that the cglF3 gene affects a mechanism stimulating cells moving to foci or affects a mechanism for coordinating early cell behavior. Mutants containing the cglF3 mutation in combination with a cglB, cglC, cglE, or cglF1 mutation had severe defects in aggregation and failed to recover from the early delay. The severity of the defects in mutants containing two cgl mutations suggests that cgl genes are critical for development. We propose that cgl genes stimulate cell movement or control specific contacts between cells during aggregation.