Biodegradation of phenol at high concentration by a novel fungal strain Paecilomyces variotii JH6.
Biodegradation of phenol at high concentration by a novel fungal strain Paecilomyces variotii JH6.
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DOI：
10.1016/j.jhazmat.2010.07.033
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发表时间：
2010-11
影响因子：
13.6
通讯作者：
Liming Wang;Yong Li;Ping Yu;Zhixiong Xie;Yunbai Luo;Yanwen Lin
中科院分区：
文献类型：
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作者：
Liming Wang;Yong Li;Ping Yu;Zhixiong Xie;Yunbai Luo;Yanwen Lin
从活性污泥中分离到一株新的苯酚降解丝状真菌JH 6，经形态学和系统发育分析鉴定为变种拟青霉（Paecilomyces variotii）。降解试验表明，该菌株能够利用浓度高达1800 mg/l的苯酚作为唯一的碳源和能源。该菌株在pH 5、37°C、葡萄糖浓度为100 mg/l时对苯酚的降解效果最好。在较宽的苯酚初始浓度范围内（100- 1800 mg/l），Haldalle的模型可以很好地拟合生长动力学数据，动力学值μmax= 0.312 h −1，Ks= 130.4 mg/l，Ki= 200 mg/l。衰变系数为0.0073h−1。菌株JH 6在其它有毒物质如间甲酚和喹啉存在下也能完全降解苯酚，而这些有毒物质在真实的含酚废水中经常存在。
A novel phenol-degrading filamentous fungus, strain JH6, was isolated from activated sludge and identified as a member of Paecilomyces variotii based on standard morphological and phylogenetic analysis. The degradation assays suggested that the strain was able to utilize phenol as the sole source of carbon and energy at concentrations up to 1800mg/l. The strain exhibited optimum phenol degradation performance with the addition of 100mg/l glucose at pH 5, 37°C. Haldane's model could be fitted to the growth kinetics data well over a wide range of initial phenol concentrations (100–1800mg/l), with kinetic values μmax=0.312h−1, Ks=130.4mg/l, and Ki=200mg/l. The decay coefficient was found to be 0.0073h−1. Complete phenol degradation by strain JH6 could be achieved in the presence of other toxicants, such as m-cresol and quinoline, which were often found in the real phenol-containing wastewater.