PHARMACEUTICAL APPLICATION OF LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY .2. ION CHROMATOGRAPHY ION-SPRAY MASS-SPECTROMETRIC CHARACTERIZATION OF ALENDRONATE
PHARMACEUTICAL APPLICATION OF LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY .2. ION CHROMATOGRAPHY ION-SPRAY MASS-SPECTROMETRIC CHARACTERIZATION OF ALENDRONATE
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DOI：
10.1016/0021-9673(94)00798-5
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发表时间：
1994-12-02
影响因子：
4.1
通讯作者：
IP, DP
中科院分区：
文献类型：
--
作者：
QIN, XZ;TSAI, EW;IP, DP
The trihydrate of alendronate sodium (MK-0217) is an important bisphosphonate drug for the treatment of a variety of bone diseases. Determination and characterization of this compound in dosage formulations is challenging since it has no chromophore, and as a highly polar and thermally labile compound, it is not amenable to electron impact mass spectrometry. Ion chromatography coupled with an ion spray mass detector (IC-ISP-MS) in the negative ionization mode was developed and applied to the characterization of this compound. Under these conditions alendronate (m/z 248, [M - H](-), M = parent alendronic acid) was readily observed. The anion can form cluster anions with acid molecules including that of the alendronic acid in the gas phase, which is a distinguishing feature of the IC-ISP-MS spectrum. IC-ISP-MS-MS study of the anion shows that cleavage of the C-P bond(s) is the dominant fragmentation pathway(s) of the anion, characteristic of its structure.
