Phosphoprotein modulation of apatite crystallization
Phosphoprotein modulation of apatite crystallization
复制标题
磷灰石结晶的磷蛋白调节
DOI：
10.1007/bf02407188
复制
发表时间：
2006
影响因子：
4.2
通讯作者：
K. Conn
中科院分区：
文献类型：
--
作者：
J. Termine;E. Eanes;K. Conn
摘要几种磷蛋白制剂（牙本质磷蛋白，大鼠切牙和胎牛磨牙牙本质磷蛋白）都抑制磷灰石生长/复制从预先存在的晶种在亚稳溶液中。晶体生长过程的两个阶段被这些磷蛋白抑制。首先，诱导了初始滞后期，这可能与种子表面现象有关。当在晶体生长反应中使用磷蛋白预包被的晶种与可溶性磷蛋白的组合时，这一时期被无限期地延长。第二，磷蛋白延长了反应阶段，其中磷酸八钙是在其转化为最终磷灰石产物之前存在的主要矿物相。预处理的磷蛋白与钙减少其抑制活性的种子晶体生长，以及对从头磷灰石在合成细胞外液的形成。胶原蛋白的存在减少了磷蛋白对从头沉淀的抑制活性，但在接种系统中对磷蛋白调节的磷灰石晶体生长没有影响。这些结果表明磷蛋白在牙本质矿化中具有潜在的调节作用。
SummarySeveral phosphoprotein preparations (phosvitin, rat incisor and fetal calf molar dentin phosphoproteins) all inhibit apatite growth/replication from pre-existing crystal seeds in metastable solutions. Two stages of the crystal growth process were inhibited by these phosphoproteins. First, an initial lag period was induced, probably associated with seed surface phenomena. This period was prolonged indefinitely when a combination of phosphoprotein precoated seeds was used together with soluble phosphoproteins in the crystal growth reaction. Second, the phosphoproteins prolonged that stage of the reaction where octacalcium phosphate is the predominant mineral phase present prior to its conversion to the final apatite product. Pretreatment of the phosphoproteins with calcium diminished their inhibitory activity to seeded crystal growth as well as towards de novo apatite formation in synthetic extracellular fluids. The presence of collagen diminished the inhibitory activity of the phosphoproteins towards de novo precipitation but had no effect on phosphoprotein-modulated apatite crystal growth in the seeded systems. These results suggest a potential regulatory role for phosphoproteins in dentin mineralization.