A SUPPRESSOR OF TBP MUTATIONS ENCODES AN RNA POLYMERASE-III TRANSCRIPTION FACTOR WITH HOMOLOGY TO TFIIB
A SUPPRESSOR OF TBP MUTATIONS ENCODES AN RNA POLYMERASE-III TRANSCRIPTION FACTOR WITH HOMOLOGY TO TFIIB
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DOI：
10.1016/0092-8674(92)90351-c
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发表时间：
1992-10-16
期刊：
影响因子：
64.5
通讯作者：
ZHOU, H
中科院分区：
文献类型：
--
作者：
BURATOWSKI, S;ZHOU, H
将S.酿酒酵母作为TATA结合蛋白（TBP）碱性区域内突变的等位基因特异性高拷贝抑制因子分离。该基因是生存所必需的，编码596个氨基酸的蛋白质。TDS4蛋白的前300个氨基酸与RNA聚合酶II转录因子TFIIB具有显著的序列相似性。然而，TDS4是RNA聚合酶III在体内和体外转录所必需的。TDS4或TBP特异性抗体与TFIIIB复合物反应，表明这两种蛋白质都是RNA聚合酶III起始复合物的组分。这些发现表明，RNA聚合酶II和III的起始机制是非常相似的，他们解释了TATA结合蛋白如何在两个系统中发挥作用。
The TDS4 gene of S. cerevisiae was isolated as an allele-specific high copy suppressor of mutations within the basic region of the TATA-binding protein (TBP). The gene is essential for viability and encodes a 596 aa protein. The first 300 aa of the TDS4 protein exhibit significant sequence similarity to the RNA polymerase II transcription factor TFIIB. However, TDS4 is required for RNA polymerase III transcription in vivo and in vitro. Antibodies specific for TDS4 or TBP react with the TFIIIB complex, indicating that both proteins are components of the RNA polymerase III initiation complex. These findings suggest that the RNA polymerase II and III initiation mechanisms are extremely similar, and they explain how the TATA-binding protein can function in both systems.