Pseudoaneuploid subpopulations detected in normal upper aerodigestive tract mucosa consistent with physiological apoptosis in normally differentiating squamous mucosa.
Pseudoaneuploid subpopulations detected in normal upper aerodigestive tract mucosa consistent with physiological apoptosis in normally differentiating squamous mucosa.
复制标题
在正常上呼吸消化道粘膜中检测到的假非整倍体亚群与正常分化的鳞状粘膜中的生理性细胞凋亡一致。
DOI：
10.1016/j.otohns.2004.04.036
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ensley,JohnF
中科院分区：
文献类型：
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作者：
El-Rayes,BassilF;Korkmaz,Hakan;Maciorowski,Zosia;Sakr,Wael;Jacobs,JohnR;Ensley,JohnF
结论：在评估使用正常人上呼吸消化道粘膜细胞作为二倍体标准品用于流式细胞术研究头颈部鳞状细胞癌DNA含量的有效性时，经常检测到假非整倍体。本研究的目的是进一步评估这些DNA含量异常遇到正常人粘膜细胞，并将其与生理apoptosis.STUDY DESIGN：32个标本的上aerodegradatory道粘膜从18个手术切除，11个新鲜尸检，和3颊刮片进行了检查的DNA含量通过流式细胞术.RESULTS：假非整倍体，范围从亚G 0/G1峰超二倍体峰增加90°光散射特性被发现在60%的这些标本。荧光显微镜检查分选的DNA假非整倍体细胞表现出细胞凋亡。结论：这意想不到的假非整倍体在正常粘膜细胞是生理性细胞凋亡，鳞状细胞分化的正常组成部分的结果。循证医学等级：B-2。
OBJECTIVES:While evaluating the validity of using normal human mucosal cells from the upper aerodigestive tract as diploid standards for DNA content studies of squamous cell cancer of head and neck by flow cytometry, pseudoaneuploidy was frequently detected. The purpose of this study was to further evaluate these DNA content abnormalities encountered in normal human mucosal cells and correlate them to physiological apoptosis.STUDY DESIGN:Thirty-two specimens of upper areodigestive tract mucosa from 18 surgical resections, 11 fresh autopsies, and 3 buccal scrapings were examined for DNA content by flow cytometry.RESULTS:Pseudoaneuploidy, which ranged from sub-G0/G1 peaks to hyperdiploid peaks with increased 90° light scattering properties was found in 60% of these specimens. Fluorescent microscopic examination of the sorted DNA pseudoaneuploid cells demonstrated cells undergoing apoptosis.CONCLUSION:This unexpected pseudoaneuploidy in normal mucosal cells was a result of physiological apoptosis, a normal component of squamous differentiation. EBM rating: B-2.