Strain-specific expression profiles of virulence genes in Helicobacter pylori during infection of gastric epithelial cells and granulocytes
Strain-specific expression profiles of virulence genes in Helicobacter pylori during infection of gastric epithelial cells and granulocytes
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DOI：
10.1016/j.micinf.2004.11.018
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发表时间：
2005-03-01
影响因子：
5.8
通讯作者：
Backert, S
中科院分区：
文献类型：
--
作者：
Gieseler, S;König, B;Backert, S
幽门螺杆菌表达多种已知的毒力相关因子，其表达可能依赖于该病原体的生态位。在这里，我们比较了H.幽门螺杆菌菌株分离自不同病理的患者。我们的目的是研究这些毒力因子在AGS胃上皮细胞和粒细胞感染过程中的协调基因表达谱。采用实时定量RT-PCR（TaqMan）方法，检测cagA、ureA、napA、katA、vacAs 1和vacAs 2等位基因mRNA表达水平。所研究基因的表达谱根据菌株而变化，并且在细菌与AGS细胞接触后主要上调或不变。相反，在与粒细胞接触时，H中的大多数基因被抑制。幽门。主要结果如下：（1）遗传多样性H. pylori表现出不同的mRNA表达谱，（ii）表达模式是菌株特异性和时间依赖性的，（iii）表达谱的调节是宿主细胞依赖性的。这些数据具有统计学意义，表明与靶细胞接触会导致病原体与其宿主之间的主动串扰。使用Taqman-PCR来分析细菌病原体的mRNA的表达，以响应不断变化的宿主环境，使我们能够以灵敏和可重复的方式识别可变的和菌株特异性的转录谱。(c)2005年，Elsevier SAS。All rights reserved.
Helicobacter pylori expresses a variety of known virulence-associated factors, whose expression is likely to be dependent on the ecological niche of this pathogen. Here, we compared the temporal changes in the level of virulence-associated gene transcription in H. pylori strains isolated from patients with different pathology. Our aim was to study the coordinated gene expression profiles of these virulence factors during infection of AGS gastric epithelial cells and granulocytes. Using real-time quantitative (TaqMan) RT-PCR, we determined the mRNA expression of cagA, ureA, napA, katA, vacAs1 and vacAs2 alleles in a time course up to 6 h. The expression profiles of the investigated genes vary according to the strain, and were mainly either upregulated or unchanged upon bacterial contact with AGS cells. In contrast, upon contact with granulocytes, the majority of the genes were repressed in H. pylori. The following major results were obtained: (i) genetically diverse H. pylori exhibit different mRNA expression profiles, (ii) the expression patterns were strain-specific and time-dependent and (iii) the regulation of expression profiles was host cell dependent. These data were statistically significant and suggest that contact with target cells leads to an active cross-talk between the pathogen and its host. The use of Taqman-PCR to analyse the expression of mRNA of a bacterial pathogen in response to a changing host environment enabled us to identify variable and strain-specific transcription profiles in a sensitive and reproducible manner. (c) 2005 Elsevier SAS. All rights reserved.