Lactobacillus rhamnosus GG alleviates β-conglycinin-induced allergy by regulating the T cell receptor signaling pathway
Lactobacillus rhamnosus GG alleviates β-conglycinin-induced allergy by regulating the T cell receptor signaling pathway
复制标题
鼠李糖乳杆菌 GG 通过调节 T 细胞受体信号通路减轻 β-伴大豆球蛋白诱导的过敏
DOI：
10.1039/d0fo02124e
复制
发表时间：
2020-12-01
期刊：
影响因子：
6.1
通讯作者：
Wang, Shuo
中科院分区：
文献类型：
--
作者：
Chen, Xiaoxu;Zhao, Xiuli;Wang, Shuo
目前，需要安全有效的方法来缓解过敏是一个重要的问题。本研究评价了鼠李糖乳杆菌GG（Lactobacillus rhamnosus GG，LGG）在减轻β-伴大豆球蛋白（beta-conglycinin，β-CG）诱导的过敏反应中的作用，并阐明了相关的分子机制。典型过敏症状和血清中炎性因子的检测结果表明，LGG干预能有效缓解β-CG诱导的小鼠过敏反应，且效果优于自然恢复（NR）。LGG干预后肠绒毛恢复，CD 4 + T细胞浸润水平降低。我们评估了LGG干预是否削弱了过敏小鼠脾细胞的增殖能力，平衡T/B细胞和Th 1/Th 2和Th 17/Treg细胞因子。转录组分析显示，LGG组与β-CG组比较，共鉴定出4106条差异表达的mRNA，LGG组与NR组比较，共鉴定出546条差异表达的mRNA。KEGG通路分析鉴定了T细胞受体（TCR）信号通路在LGG干预后显著富集，并且通过qPCR分析进一步验证了上调的Ifnar 2和下调的Tgfbr 2、Il 13 r2和Il 4 ra。因此，上述结果充分揭示了LGG在缓解β-CG诱发过敏中的重要作用。
Currently, the need for safe and effective methods for relieving allergies is an important concern. In this study, we evaluated the role of Lactobacillus rhamnosus GG (LGG) in alleviating beta-conglycinin (beta-CG)-induced allergies and elucidated the related molecular mechanisms. Typical allergy symptoms and inflammatory factors in the serum showed that LGG intervention effectively alleviated beta-CG induced allergy in mice, which was better than natural recovery (NR). Intestinal villi were restored and lower levels of CD4+ T cells infiltrated after LGG intervention. We evaluated whether LGG intervention weakened the proliferation ability of the spleen cells of allergic mice, balancing between T/B cells and Th1/Th2 and Th17/Treg cytokines. Transcriptome analysis revealed that 4106 differentially expressed mRNAs were identified by comparing the LGG group and beta-CG group, and 546 differentially expressed mRNAs were identified by comparing the LGG group and NR group. KEGG pathway analysis identified that the T cell receptor (TCR) signaling pathway was significantly enriched upon LGG intervention, and the upregulated Ifnar2 and the downregulated Tgfbr2, Il13r2 and Il4ra were further validated by qPCR analysis. Therefore, the above results fully revealed the important role of LGG in alleviating beta-CG-induced allergies.