A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes
A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes
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DOI：
10.1016/j.mod.2004.04.016
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发表时间：
2004-07-01
影响因子：
2.6
通讯作者：
Kondoh, H
中科院分区：
文献类型：
--
作者：
Furutani-Seiki, M;Sasadoa, T;Kondoh, H
在Medaka进行了大规模的突变筛选，以确定在不同发育过程中发挥作用的基因。在来自ENU治疗的创始人男性的纯合子F3子代中发现了突变。除了活胚胎的形态检查外，还使用了其他方法来检测器官发生和特定细胞过程中的异常，包括生殖细胞迁移、神经束形成。感觉器官分化和DNA修复。在已鉴定的2031个胚胎致死突变中，312个导致器官发生缺陷的突变被选作进一步分析。在这些突变中，126个突变被描述为遗传特征，并被归类为105个基因。Medaka和斑马鱼发育的相似性促进了突变表型的比较，这表明Medaka的许多突变导致了迄今为止在斑马鱼中未记录的独特表型。即使这两种鱼的突变导致了类似的表型，如独眼针头或降落伞，在Medaka中也发现了比斑马鱼更多的基因，后者在突变时产生了相同的表型。这些观察表明，许多Medaka突变体代表了新的基因，因此是对斑马鱼突变体集合的重要补充，事实证明，这些突变体对于探索发育中的基因组功能非常有价值。(C)2004年，爱思唯尔爱尔兰有限公司出版。
A large-scale mutagenesis screen was performed in Medaka to identify genes acting in diverse developmental processes. Mutations were identified in homozygous F3 progeny derived from ENU-treated founder males. In addition to the morphological inspection of live embryos, other approaches were used to detect abnormalities in organogenesis and in specific cellular processes, including germ cell migration, nerve tract formation. sensory organ differentiation and DNA repair. Among 2031 embryonic lethal mutations identified, 312 causing defects in organogenesis were selected for further analyses. From these, 126 mutations were characterized genetically and assigned to 105 genes. The similarity of the development of Medaka and zebrafish facilitated the comparison of mutant phenotypes, which indicated that many mutations in Medaka cause unique phenotypes so far unrecorded in zebrafish. Even when mutations of the two fish species cause a similar phenotype such as one-eyed-pinhead or parachute, more genes were found in Medaka than in zebrafish that produced the same phenotype when mutated. These observations suggest that many Medaka mutants represent new genes and, therefore, are important complements to the collection of zebrafish mutants that have proven so valuable for exploring genomic function in development. (C) 2004 Published by Elsevier Ireland Ltd.