Continuous i.c.v. infusion of brain-derived neurotrophic factor modifies hypothalamic-pituitary-adrenal axis activity, locomotor activity and body temperature rhythms in adult male rats
Continuous i.c.v. infusion of brain-derived neurotrophic factor modifies hypothalamic-pituitary-adrenal axis activity, locomotor activity and body temperature rhythms in adult male rats
复制标题
DOI：
10.1016/j.neuroscience.2005.12.028
复制
发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Givalois, L
中科院分区：
文献类型：
--
作者：
Naert, G;Ixart, G;Givalois, L
脑源性神经营养因子（Brain-derived neurotrophic factor，BDNF）是神经生长因子家族中的一种神经营养因子，参与多种神经元的分化和存活。它还参与成年大鼠的神经保护和神经元可塑性。我们以前的研究表明，一个单一的脑源性神经营养因子注射修改下丘脑-垂体-肾上腺轴的活动在成年雄性大鼠。为了研究慢性脑源性神经营养因子给药对某些生理参数的影响，成年大鼠植入渗透微泵，在侧脑室连续14天（12 μ g/天/大鼠）提供脑源性神经营养因子。用原位杂交法测定mRNA水平，用放射免疫法测定肽含量和血浆激素浓度。动物还配备了遥测发射器，以研究运动活动和温度节律的变化，因为已知下丘脑-垂体-肾上腺轴调节这两个参数。在下丘脑中，连续脑源性神经营养因子处理增加：（i）室旁核、视上核和视交叉上核中促肾上腺皮质激素释放激素和垂体后叶素-加压素的mRNA稳态水平;（ii）通过原位杂交检测这些核中促肾上腺皮质激素释放激素和垂体后叶素-加压素mRNA信号的表面，和（iii）促肾上腺皮质激素释放激素和垂体后叶素-加压素含量。血浆促肾上腺皮质激素和皮质酮浓度分别降低和升高。最后，这种治疗增加了每日运动活动和温度，并引起了一些昼夜节律的扰动。慢性脑源性神经营养因子给药后获得的这些结果扩展了脑源性神经营养因子参与下丘脑-垂体-肾上腺轴调节的数据，并说明了其对运动和温度节律的影响。它们还允许证明脑源性神经营养因子对下丘脑-垂体-肾上腺轴的调节根据脑源性神经营养因子施用模式（即急性注射或慢性施用）而不同。(C)2006由Elsevier Ltd代表IBRO出版。
Brain-derived neurotrophic factor is a neurotrophin belonging to the nerve growth factor family, which is involved in the differentiation and survival of many types of neurons. It also participates in neuroprotection and neuronal plasticity in adult rats. Our previous studies showed that a single brain-derived neurotrophic factor injection modifies hypothalamic-pituitary-adrenal axis activity in adult male rats. To investigate the effect of chronic brain-derived neurotrophic factor administration on some physiological parameters, adult rats were implanted with osmotic micropumps to deliver brain-derived neurotrophic factor continuously for 14 days in the lateral ventricle (12 mu g/day/rat). mRNA levels were evaluated by in situ hybridization analysis, peptide contents and plasma hormone concentrations by radioimmunoassay. Animals were also equipped with telemetric transmitters to study locomotor activity and temperature rhythms modifications, since hypothalamic-pituitary-adrenal axis is known to modulate these two parameters. Decreased body weight was used as a control of brain-derived neurotrophic factor access to hypothalamic areas as already documented.In the hypothalamus the continuous brain-derived neurotrophic: factor treatment increases: (i) the mRNA steady state levels of corticotropin releasing hormone and arginin-vasopressin in the paraventricular nucleus, the supraoptic nucleus, and the suprachiasmatic nucleus; (ii) the surface of corticotropin releasing hormone and arginin-vasopressin mRNA signals in these nuclei as detected by in situ hybridization, and (iii) the corticotropin releasing hormone and arginin-vasopressin contents. The plasma concentrations of adrenocorticotropic hormone and corticosterone were decreased and increased, respectively. Finally, this treatment increased daily locomotor activity and temperature, and provoked some circadian perturbations. These results obtained after chronic brain-derived neurotrophic factor administration extend data on the brain-derived neurotrophic factor involvement in the hypothalamic-pituitary-adrenal axis regulation and illustrate its effects on the locomotor and temperature rhythms. They also allow demonstrating that the regulation of the hypothalamic-pituitary-ad renal axis by brain-derived neurotrophic factor differs according to the brain-derived neurotrophic factor administration mode, i.e. acute injection or chronic administration. (C) 2006 Published by Elsevier Ltd on behalf of IBRO.