Insights into the distribution and abundance of the ubiquitous candidatus Saccharibacteria phylum following tag pyrosequencing.
Insights into the distribution and abundance of the ubiquitous candidatus Saccharibacteria phylum following tag pyrosequencing.
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DOI：
10.1038/srep03957
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发表时间：
2014-02-04
影响因子：
4.6
通讯作者：
Neilan B
中科院分区：
文献类型：
--
作者：
Ferrari B;Winsley T;Ji M;Neilan B
Candidatus Saccharomyces细菌门（以前称为Candidate Division TM7）是一个高度普遍存在的门，其16S rRNA基因序列在土壤、沉积物、废水和动物以及许多临床环境中均有报道。在这里，应用两个分类群特异性引物对环境和人类相关的样品，使用条形码标签焦磷酸测序揭示了两个新的分支，这个门存在，我们建议，该司由2个单系和2个多系分支。TM7生态调查显示，高比例（58%）的杂草类型是样品特有的，很少有广泛分布的，那些最广泛分布的都属于亚组3。此外，50%的相对丰富的物种类型也是亚类3成员。群落分析表明，尽管存在着高比例的独特类型，特定组的样品仍然怀有类似的TM7社区与样品聚类在一起。缺乏相对丰富的亚门1，2和4的模式和非常少的世界性成员的存在，不仅突出了该门的特定地点的性质，但提供了洞察为什么大多数研究TM7已经偏向亚门3。
The phylum candidatus Saccharibacteria formerly known as Candidate Division TM7 is a highly ubiquitous phylum with 16S rRNA gene sequences reported in soils, sediments, wastewater and animals, as well as a host of clinical environments. Here, the application of two taxon-specific primers on environmental and human-associated samples using bar-coded tag pyrosequencing revealed two new clades for this phylum to exist and we propose that the division consists of 2 monophyletic and 2 polyphyletic clades. Investigation into TM7 ecology revealed that a high proportion (58%) of phylotypes were sample specific, few were widely distributed and of those most widely distributed all belonged to subdivision 3. Additionally, 50% of the most relatively abundant phylotypes observed were also subdivision 3 members. Community analysis showed that despite the presence of a high proportion of unique phylotypes, specific groups of samples still harbor similar TM7 communities with samples clustering together. The lack of relatively abundant phylotypes from subdivisions 1, 2 and 4 and the presence of very few cosmopolitan members' highlights not only the site specific nature of this phylum but provides insight into why the majority of studies into TM7 have been biased towards subdivision 3.