Construction of multifunctional proteins for tissue engineering: Epidermal growth factor with collagen binding and cell adhesive activities
Construction of multifunctional proteins for tissue engineering: Epidermal growth factor with collagen binding and cell adhesive activities
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DOI：
10.1016/j.jbiotec.2008.09.011
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发表时间：
2009-01-01
影响因子：
4.1
通讯作者：
Kobatake, Eiry
中科院分区：
文献类型：
--
作者：
Imen, Elloumi Hannachi;Nakamura, Makiko;Kobatake, Eiry
基于油、天然和聚合物支架的不同技术的发展为不同仿生材料的设计提供了有益的参考。这些方法。然而.需要对生物材料进行化学或物理改性的补充步骤。为了避免这些步骤，在本研究中，我们构建了一种新的多功能蛋白，它可以很容易地固定在疏水表面上，同时有助于增强细胞在胶原基质上的特异性粘附和增殖。将胶原结合结构域融合到先前构建的蛋白质上，该蛋白质具有融合到允许细胞粘附的疏水肽上的表皮生长因子。新的融合蛋白。命名为fnCBD-ERE-EGF在大肠杆菌中产生。并研究其与胶原结合和促进细胞增殖的能力。fnCBD-ERE-EGF显示出与相应的未融合蛋白质相当的胶原结合和细胞生长促进活性。本研究中获得的结果还建议使用fnCBD-ERE-EGF作为设计基于多功能ECM结合生长因子的材料的替代方案。(c)2008 Elsevier B.V.保留所有权利。
The development of different techniques based oil natural and polymeric scaffolds are useful for the design of different biomimetic materials. These approaches. however. require Supplementary steps for the chemical or physical modification of the biomaterial. To avoid Such steps, in the present study, we constructed a new multifunctional protein that can he easily immobilized onto hydrophobic surfaces, and at the same time helps enhance specific cell adhesion and proliferation onto collagen Substrates. A collagen binding domain was Fused to a previously constructed protein, which had an epidermal growth factor fused to a hydrophobic peptide that allows for cell adhesion. The new fusion protein. designated fnCBD-ERE-EGF is produced in Escherichia coli. and its abilities to bind to collagen and promote cell proliferation were investigated. fnCBD-ERE-EGF was shown to keep both collagen binding and cell growth-promoting activities comparable to those of the corresponding unfused proteins. The results obtained in this study also Suggest the use of a fnCBD-ERE-EGF as an alternative for the design of multifunctional ECM-bound growth factor based materials. (c) 2008 Elsevier B.V. All rights reserved.