Coordinate regulation of the biosynthesis of ATP-citrate lyase and malic enzyme during adipocyte differentiation. Studies on 3T3-L1 cells.
Coordinate regulation of the biosynthesis of ATP-citrate lyase and malic enzyme during adipocyte differentiation. Studies on 3T3-L1 cells.
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DOI：
10.1016/s0021-9258(17)42920-6
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发表时间：
1984-04
期刊：
影响因子：
--
通讯作者：
L. S. Wise;H. Sul;C. Rubin
中科院分区：
文献类型：
--
作者：
L. S. Wise;H. Sul;C. Rubin
研究了3 T3-L1前脂肪细胞向脂肪细胞分化过程中两种脂肪生成酶的合成和降解。在脂肪细胞发育过程中，苹果酸酶的活性和质量均呈一个数量级的上升，在成熟脂肪细胞中，苹果酸酶占胞浆蛋白的0.3%。类似地，ATP-柠檬酸裂解酶的活性和量在脂肪转化期间增加约7倍。在前脂肪细胞中，两种酶的相对合成率均小于或等于0.02%，但随着细胞开始分化，两种酶的相对合成率急剧增加。苹果酸酶和ATP-柠檬酸裂解酶的合成在3 T3-L1脂肪细胞的最大稳态速率分别为13倍和8倍，比在前脂肪细胞的基础率。与此相反，苹果酸酶（67小时）和ATP-柠檬酸裂解酶（47小时）的半衰期在脂肪细胞发育过程中没有改变。因此，酶合成的加速速率解释了两种脂肪生成酶的分化依赖性积累。在脂肪细胞分化过程中，苹果酸酶、ATP-柠檬酸裂解酶和脂肪酸合成酶生物合成的速率增加以高度协调的方式表达，并且与这些酶的可翻译mRNA水平的平行升高相关。
The biosynthesis and degradation of two lipogenic enzymes were studied during the differentiation of 3T3-L1 preadipocytes into adipocytes. The activity and mass of malic enzyme, rose by an order of magnitude during adipocyte development and the enzyme accounted for 0.3% of the cytosol protein in mature fat cells. Similarly, the activity and amount of ATP-citrate lyase increased approximately 7-fold during the adipose conversion. The relative rates of synthesis of the two enzymes were less than or equal to 0.02% in preadipocytes, but increased sharply as the cells began to differentiate. Maximal steady state rates of malic enzyme and ATP-citrate lyase synthesis in 3T3-L1 adipocytes were 13- and 8-fold higher, respectively, than the basal rates in preadipocytes. In contrast, the half-lives of malic enzyme (67 h) and ATP-citrate lyase (47 h) were not altered during adipocyte development. Thus, accelerated rates of enzyme synthesis account for the differentiation-dependent accumulation of the two lipogenic enzymes. Increased rates of malic enzyme, ATP-citrate lyase, and fatty acid synthetase biosynthesis are expressed in a highly coordinated manner during adipocyte differentiation and are associated with parallel elevations in the levels of translatable mRNAs for these enzymes.