Filamentous phage SW1 is active and influences the transcriptome of the host at high-pressure and low-temperature
Filamentous phage SW1 is active and influences the transcriptome of the host at high-pressure and low-temperature
复制标题
丝状噬菌体SW1在高压低温下具有活性并影响宿主的转录组
DOI：
10.1111/1758-2229.12388
复制
发表时间：
2016-06-01
影响因子：
3.3
通讯作者：
Xiao, Xiang
中科院分区：
文献类型：
--
作者：
Jian, Huahua;Xiong, Lei;Xiao, Xiang
作为地球上最丰富的生物实体，病毒参与了全球海洋地球化学循环，并已被证明在深海生态系统的整体运作中发挥重要作用。然而，深海病毒是否以及如何影响其细菌宿主的生理学，人们知之甚少。以前，丝状噬菌体SW 1被鉴定为深海细菌Shewanella piezotolerans WP 3，其分离自西太平洋上层沉积物。在这项研究中，通过透射电子显微镜和逆转录定量聚合酶链反应显示噬菌体SW 1在原位环境条件（20 MPa和4摄氏度）下具有活性。进一步的比较分析表明，SW 1对其宿主的生长和转录组有显著的影响。负责基本细胞活动的基因的转录，包括转录/翻译装置、精氨酸合成、嘌呤代谢和鞭毛马达，被噬菌体下调。我们的研究结果首次表征了高压和低温条件下的噬菌体-宿主相互作用，这表明噬菌体调整了宿主的能量利用策略，以改善在深海环境中的生存。
As the most abundant biological entities on the planet, viruses are involved in global biogeochemical cycles, and they have been shown to play an important role in the overall functioning of the deep-sea ecosystem. Nevertheless, little is known about whether and how deep-sea viruses affect the physiology of their bacterial hosts. Previously, the filamentous phage SW1 was identified in the bathypelagic bacterium Shewanella piezotolerans WP3, which was isolated from the upper sediment of West Pacific ocean. In this study, phage SW1 was shown to be active under in situ environmental conditions (20 MPa and 4 degrees C) by transmission electron microscopy and reverse-transcription quantitative polymerase chain reaction. Further comparative analysis showed that SW1 had a significant influence on the growth and transcriptome of its host. The transcription of genes responsible for basic cellular activities, including the transcriptional/translational apparatus, arginine synthesis, purine metabolism and the flagellar motor, were down-regulated by the phage. Our results present the first characterization of a phage-host interaction under high-pressure and low-temperature conditions, which indicated that the phage adjusted the energy utilization strategy of the host for improved survival in deep-sea environments.