Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood
Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood
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DOI：
10.1016/j.neures.2009.10.007
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发表时间：
2010-02-01
影响因子：
2.9
通讯作者：
Shibata, Shigenobu
中科院分区：
文献类型：
--
作者：
Ebisawa, Takashi;Numazawa, Kahori;Shibata, Shigenobu
昼夜节律紊乱与睡眠和情绪障碍等人类疾病有关。然而，对人体内源性昼夜节律的研究既费力又费时，这阻碍了疾病的阐明。据报道，周围组织与视交叉上核（生物钟的中心）一样表现出昼夜节律。我们尝试使用从单次静脉血采集中获得的培养外周血单核细胞 (PBMC) 来研究人类昼夜节律。激活的人类 PBMC 显示出时钟基因表达的自我维持昼夜节律，这表明它们可用于研究人类内源性昼夜节律。 (C) 2009 Elsevier Ireland Ltd 和日本神经科学学会。版权所有。
Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.