Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites
Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites
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DOI：
10.1101/gad.1391006
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发表时间：
2006-04-01
影响因子：
10.5
通讯作者：
Jan, YN
中科院分区：
文献类型：
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作者：
Parrish, JZ;Kim, MD;Jan, YN
树突分枝模式是神经元功能的关键决定因素。为了探索树突模式形成中转录调控的基础，我们利用RNA干扰(RNAi)技术筛选了730个转录调控因子，并鉴定了78个基因，这些基因与果蝇I类da神经元的树突状突起构型有关。这些转录调控因子大多影响树突形态，但不改变I类树突树枝形成(Da)神经元的数量，主要分为三类。A组基因既控制初级树突伸展，也控制侧枝，从而控制整个树突场。A组中有19个基因促进了树枝的形成，而其他20个基因限制了树突的覆盖。B组基因似乎平衡了树突状突起和分支。19个B组基因的功能是促进分枝而不是生长，另外两个基因的作用相反。最后，10个C组基因对单个I类DA神经元的树突状突起的传递至关重要。因此，多个遗传程序操作来校准树突的覆盖范围，协调初级和次级分支的细化，并以适当的方向布置这些树突分支。
Dendrite arborization patterns are critical determinants of neuronal function. To explore the basis of transcriptional regulation in dendrite pattern formation, we used RNA interference (RNAi) to screen 730 transcriptional regulators and identified 78 genes involved in patterning the stereotyped dendritic arbors of class I da neurons in Drosophila. Most of these transcriptional regulators affect dendrite morphology without altering the number of class I dendrite arborization (da) neurons and fall primarily into three groups. Group A genes control both primary dendrite extension and lateral branching, hence the overall dendritic field. Nineteen genes within group A act to increase arborization, whereas 20 other genes restrict dendritic coverage. Group B genes appear to balance dendritic outgrowth and branching. Nineteen group B genes function to promote branching rather than outgrowth, and two others have the opposite effects. Finally, 10 group C genes are critical for the routing of the dendritic arbors of individual class I da neurons. Thus, multiple genetic programs operate to calibrate dendritic coverage, to coordinate the elaboration of primary versus secondary branches, and to lay out these dendritic branches in the proper orientation.