Electrophysiological properties of NG2(+) cells: Matching physiological studies with gene expression profiles.
Electrophysiological properties of NG2(+) cells: Matching physiological studies with gene expression profiles.
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DOI：
10.1016/j.brainres.2015.09.010
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发表时间：
2016-05-01
期刊：
影响因子：
2.9
通讯作者：
Bergles DE
中科院分区：
文献类型：
--
作者：
Larson VA;Zhang Y;Bergles DE
NG2+神经胶质细胞是中枢神经系统中形成髓鞘少突胶质细胞的非神经细胞的动态群体。这些细胞表达大量的离子通道和神经递质受体，这使得它们具有复杂的电生理特征，这在神经胶质细胞中是独一无二的。尽管对这些细胞的电生理特性进行了广泛的分析，但对它们所表达的通道和受体的分子特性知之甚少。针对NG2+细胞的新的RNA-Seq数据集的产生为探索不同的基因如何影响这些前体细胞的生理特性提供了手段。在本文中，我们对纯化的NG2+细胞的RNA-Seq转录分析结果与以往对这些细胞的生理和分子研究结果进行了系统的比较，以确定哺乳动物大脑中NG2+细胞表达的离子通道和神经递质受体的补充性，并讨论了这些细胞独特的生理特性的潜在意义。
NG2+ glial cells are a dynamic population of non-neuronal cells that give rise to myelinating oligodendrocytes in the central nervous system. These cells express numerous ion channels and neurotransmitter receptors, which endow them with a complex electrophysiological profile that is unique among glial cells. Despite extensive analysis of the electrophysiological properties of these cells, relatively little was known about the molecular identity of the channels and receptors that they express. The generation of new RNA-Seq datasets for NG2+ cells has provided the means to explore how distinct genes contribute to the physiological properties of these progenitors. In this review, we systematically compare the results obtained through RNA-Seq transcriptional analysis of purified NG2+ cells to previous physiological and molecular studies of these cells to define the complement of ion channels and neurotransmitter receptors expressed by NG2+ cells in the mammalian brain and discuss the potential significance of the unique physiological properties of these cells.