A map of the interactome network of the metazoan C-elegans
A map of the interactome network of the metazoan C-elegans
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DOI：
10.1126/science.1091403
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发表时间：
2004-01-23
期刊：
影响因子：
56.9
通讯作者：
Vidal, M
中科院分区：
文献类型：
--
作者：
Li, SM;Armstrong, CM;Vidal, M
为了研究蛋白质-蛋白质相互作用(或“相互作用组”)网络如何与多细胞功能相关，我们绘制了秀丽线虫相互作用组网络的一大部分。从后生动物特有蛋白质的子集开始，从高通量酵母双杂交(HT=Y2H)筛查中鉴定出4000多个相互作用。独立的共亲和纯化分析实验验证了该Y2H数据集的整体质量。连同已经描述的Y2H相互作用和在Silico中预测的相互作用，当前版本的蠕虫相互作用组(WI5)图包含类似于5500个相互作用。这种相互作用组网络的拓扑和生物学特征，以及它与表现组和转录组数据集的集成，导致了许多生物学假说。
To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains similar to5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.