Magnetic-field-assisted photothermal therapy of cancer cells using Fe-doped carbon nanoparticles
Magnetic-field-assisted photothermal therapy of cancer cells using Fe-doped carbon nanoparticles
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DOI：
10.1117/1.jbo.17.1.018003
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发表时间：
2012-01-01
影响因子：
3.5
通讯作者：
Mohanty, Samarendra K.
中科院分区：
文献类型：
--
作者：
Gu, Ling;Vardarajan, Vijaylakshmi;Mohanty, Samarendra K.
纳米粒子辅助的光热疗法为摧毁癌细胞提供了一种解决方案，而不会对原本健康的细胞造成重大附带损害。然而，将所需注射的纳米颗粒数量降至最低是一个重大挑战。在这里，我们介绍了磁性碳纳米颗粒(MCNPs)的使用，通过施加外部磁场将它们定位在所需的区域，并用近红外激光照射靶向癌细胞。利用超声喇叭空化场中产生的等离子体放电，在苯中制备了MCNP。磁性测量证实，用铁电极掺杂碳纳米管使碳纳米管具有铁磁性。透射电子显微镜测量表明，这些MCNP的尺寸分布在5-10 nm范围内。对于光热照射，在倒置荧光显微镜下，可调谐的连续波钛宝石激光弱聚焦到细胞单层上。研究了不同类型细胞对光热照射的响应。形态改变和碘化丙啶荧光包合实验证实了MCNPs和激光共同作用下的细胞死亡。我们的研究结果表明，基于MCNP的光热疗法是一种很有前途的远程引导光热疗法的方法。(C)2012年，光学仪器工程师学会。[DOI：10.1117/1.JBO.17.1.018003]
Photothermal therapy with assistance of nanoparticles offers a solution for the destruction of cancer cells without significant collateral damage to otherwise healthy cells. However, minimizing the required number of injected nanoparticles is a major challenge. Here, we introduce the use of magnetic carbon nanoparticles (MCNPs), localizing them in a desired region by applying an external magnetic-field, and irradiating the targeted cancer cells with a near-infrared laser beam. The MCNPs were prepared in benzene, using an electric plasma discharge, generated in the cavitation field of an ultrasonic horn. The CNPs were made ferromagnetic by use of Fe-electrodes to dope the CNPs, as confirmed by magnetometry. Transmission electron microscopy measurements showed the size distribution of these MCNPs to be in the range of 5 to 10 nm. For photothermal irradiation, a tunable continuous wave Ti: Sapphire laser beam was weakly focused on to the cell monolayer under an inverted fluorescence microscope. The response of different cell types to photothermal irradiation was investigated. Cell death in the presence of both MCNPs and laser beam was confirmed by morphological changes and propidium iodide fluorescence inclusion assay. The results of our study suggest that MCNP based photothermal therapy is a promising approach to remotely guide photothermal therapy. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.1.018003]