Extraction of a water-soluble polysaccharide fraction from lentils and its potential application in acidified protein dispersions
Extraction of a water-soluble polysaccharide fraction from lentils and its potential application in acidified protein dispersions
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DOI：
10.1016/j.foodhyd.2021.106740
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发表时间：
2021-03-18
影响因子：
10.7
通讯作者：
Nakamura, Akihiro
中科院分区：
文献类型：
--
作者：
Nomura, Kei;Sakai, Madoka;Nakamura, Akihiro
研究了从扁豆纤维中提取水溶性扁豆多糖的工艺条件。考察了温度、pH和提取时间的影响，确定了获得高分子量多糖的最佳条件。最佳提取温度和时间组合为120 ？加热90分钟。在此条件下，pH为4时产率高达23.3%，pH为8时产率高达19.6%。此外，进行了两种不同的提取，均为90分钟，120 ？C，一个pH为4 (SLPS4)，另一个pH为8 (SLPS8)，组分的组成和分子质量表征。SLPS4和SLPS8的重量平均分子质量分别为830 kg/mol和1130 kg/mol。虽然SLPS4和SLPS8表现出相同的糖键，但它们的组成不同：SLPS4含有葡萄糖52.7%，阿拉伯糖27.6%，半乳糖醛酸6.0%，而SLPS8含有阿拉伯糖49.8%，葡萄糖19.1%，半乳糖醛酸13.2%。在扫描探针显微镜下观察到多糖具有多分支结构，糖链从分子中心向周围延伸。在含3% （w/w）脱脂乳固体的酸化乳体系中，这两种slps都被用作稳定剂。当浓度为0.4% （w/w）时，SLPS4和SLPS8在pH为3.6 ~ 4.4或3.8 ~ 4.4的范围内均能保持细颗粒的单峰分布。这项工作证明了从扁豆多糖中创造一种增值成分的潜力。
The extraction conditions of a water-soluble lentil polysaccharide fraction (SLPS) from lentil fibers were examined. The effect of temperature, pH, and time of extraction was evaluated, to determine optimal conditions resulting in a high yield of high molecular mass polysaccharides. The optimal extraction temperature and time combination was 120 ?C for 90 min. Under these conditions, the yield was as high as 23.3% at pH 4 and 19.6% at pH 8. Further, two different extractions were carried out, both for 90 min at 120 ?C, one at pH 4 (SLPS4) and the second at pH 8 (SLPS8), and the fractions characterized for composition and molecular mass. The weight average molecular mass (Mw) of the main component in the fraction was 830 kg/mol for SLPS4 and 1130 kg/mol for SLPS8. Albeit SLPS4 and SLPS8 showed the same sugar linkages, their composition was different: SLPS4 contained, glucose 52.7%, arabinose 27.6%, and galacturonic acid 6.0%, whereas SLPS8 contained arabinose 49.8%, glucose 19.1%, and galacturonic acid 13.2%. The polysaccharides were observed by a scanning probe microscope and showed a characteristic multi-branched structure, with sugar chains extending from the center to the periphery of the molecule. Both SLPSs were used as stabilizers in an acidified milk system, containing 3% (w/w) non-fat milk solids. At a concentration of 0.4% (w/w) both SLPS4 and SLPS8 were able to maintain a monomodal distribution of fine particles in the range of pH between 3.6?4.4 or 3.8?4.4, for SLPS4 and SLPS8, respectively. This work demonstrates the potential for the creation of a value added ingredient from lentil polysaccharide.