Immunobiological properties of a recombinant simian retrovirus-1 envelope protein and a neutralizing monoclonal antibody directed against it.
Immunobiological properties of a recombinant simian retrovirus-1 envelope protein and a neutralizing monoclonal antibody directed against it.
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重组猿猴逆转录病毒 1 包膜蛋白和针对该蛋白的中和单克隆抗体的免疫生物学特性。
DOI：
10.1016/0161-5890(91)90045-l
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发表时间：
1991
影响因子：
3.6
通讯作者：
Benjamini,E
中科院分区：
文献类型：
--
作者：
Werner,LL;Torres,JV;Leung,CY;Kwang,HS;Malley,A;Benjamini,E
我们先前报道，猴(D型)逆转录病毒1型(SRV-1)包膜区的147-162位氨基酸构成了小鼠和恒河猴中和抗体结合的抗原点。针对SRV-2的中和抗体指向不同的区域，包括SRV-2的残基96-102。本文报道了SRV-1重组包膜蛋白(REP)及其免疫小鼠脾细胞产生的单抗杂交瘤细胞系C11B8的活性。纯化的C11B8单抗可与SRV-1rep结合，也可与SRV-1和SRV-2结合。然而，该单抗在体外表现出毒株特异性，能够中和SRV-1感染。因此，C11B8中和了SRV-1感染，但无法中和其他四种已知的病毒血清型。C11B8还与代表142-167残基的SRV-1合成肽结合，该合成肽包含先前定义的识别SRV-1中和抗体的抗原位点。本文还包含了SRV-1 rep结构与SRV-1与细胞受体结合的证据。这表现在SRV-1rep与SRV-1竞争(但不与SRV-2)竞争并在体外抑制其感染性的能力。此外，SRV-1rep在体外可抑制C11B8对SRV-1感染的中和活性。SRV-1 rep对SRV-2中和抗体无抑制作用，以上结果表明，在SRV感染过程中，中和抗体水平产生的免疫是特定毒株所特有的，涉及对每个毒株特有的包膜序列的识别。
We previously reported that an area encompassing amino acids 147–162 of the envelope region of the simian (type D) retrovirus serotype 1 (SRV-1) constitutes an antigenic site for the binding of murine and rhesus neutralizing antibodies. Neutralizing antibodies to SRV-2 are directed to a different area, encompassing residues 96–102 of SRV-2. This paper presents data on the activity of an SRV-1 recombinant envelope protein (rEP) and of monoclonal hybridoma cell line, C11B8, produced from murine spleen cells immunized with SRV-1 rEP. Purified monoclonal antibodies from C11B8 bind to the SRV-1 rEP and to both SRV-1 and SRV-2. However, the monoclonal antibody exhibits strain specificity in the capacity to neutralize SRV-1 infectionin vitro. Thus, C11B8 neutralizes SRV-1 infection but fails to neutralize four other known serotypes of the virus. C11B8 also binds to an SRV-1 synthetic peptide representing residues 142–167, which encompasses the previously defined antigenic site of recognition for neutralizing antibodies to SRV-1.This paper also contains evidence that the SRV-1 rEP construct binds the site for SRV-1 attachment to the cell receptor. This is indicated by the ability of SRV-1 rEP to compete with SRV-1 (but not with SRV-2) and inhibit its infectivityin vitro. In addition, SRV-1 rEP inhibits the neutralizing activity of C11B8 against SRV-1 infectionin vitro. SRV-1 rEP has no inhibitory effect on rhesus neutralizing antibodies to SRV-2.Taken together, the above findings indicate that immunity conferred at the level of neutralizing antibodies during SRV infection is strain-specific and involves the recognition of envelope sequences unique to each strain.