Hyperspectral Three-Dimensional Absorption Imaging Using Snapshot Optical Tomography
Hyperspectral Three-Dimensional Absorption Imaging Using Snapshot Optical Tomography
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DOI：
10.1103/physrevapplied.18.034055
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发表时间：
2022-09-21
影响因子：
4.6
通讯作者：
Sung, Yongjin
中科院分区：
文献类型：
--
作者：
Juntunen, Cory;Abramczyk, Andrew R.;Sung, Yongjin
高光谱成像(HSI)记录了不同波长的一系列二维(2D)图像，以提供每个像素的化学指纹。结合HSI和层析数据采集方法，我们可以获得样品在三维(3D)空间中每个点的化学指纹。由于需要扫描波长和旋转光束或样品，所谓的3D HSI通常存在成像吞吐量低的问题。在本文中，我们提出了一种光学系统，它可以在与传统HSI系统相同的吞吐量下捕获样品的整个四维(4D；即3D结构和一维谱)数据集。我们的系统结合了快照投影光学层析成像(SPOT)和傅里叶变换光谱学(FTS)，SPOT通过一次快照收集多个投影图像，傅里叶变换光谱通过收集和处理一系列干涉图图像获得更高的光谱分辨率。利用这一高光谱斑点系统，我们对染色的聚苯乙烯微珠、含氧红细胞和脱氧红细胞的体积吸光度进行了成像。本文所展示的四维光学系统为复杂显微样品的高通量化学成像提供了一种工具。
Hyperspectral imaging (HSI) records a series of two-dimensional (2D) images for different wavelengths to provide the chemical fingerprint at each pixel. Combining HSI with a tomographic data-acquisition method, we can obtain the chemical fingerprint of a sample at each point in three-dimensional (3D) space. The so-called 3D HSI typically suffers from low imaging throughput due to the requirement of scanning the wavelength and rotating the beam or sample. In this paper, we present an optical system that captures the entire four-dimensional (4D; i.e., 3D structure and one-dimensional spectrum) data set of a sample with the same throughput of conventional HSI systems. Our system works by combining snapshot projection optical tomography (SPOT), which collects multiple projection images with a single snapshot, and Fourier-transform spectroscopy (FTS), which results in superior spectral resolution by collecting and processing a series of interferogram images. Using this hyperspectral SPOT system, we image the volumetric absorbance of dyed polystyrene microbeads, oxygenated red blood cells (RBCs), and deoxygenated RBCs. The 4D optical system demonstrated in this paper provides a tool for high-throughput chemical imaging of complex microscopic specimens.