Effects of a variety of cytokines and inducing agents on vascular permeability factor mRNA levels in U937 cells.
Effects of a variety of cytokines and inducing agents on vascular permeability factor mRNA levels in U937 cells.
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DOI：
10.1016/s0006-291x(05)81103-9
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发表时间：
1991-10
影响因子：
3.1
通讯作者：
G. Dolecki;D. Connolly
中科院分区：
文献类型：
--
作者：
G. Dolecki;D. Connolly
血管通透性因子（VPF）是一种约40 kDa的二硫键连接的二聚体糖蛋白，具有增加血管通透性、内皮细胞生长和血管生成的活性。对VPF基因调控知之甚少。在这项研究中，我们研究了各种细胞因子和诱导剂对单核细胞样U937细胞系中VPF mRNA水平的影响。转化生长因子β 1在4小时后引起VPF mRNA水平增加1.8倍，随后在18小时下降至基础水平。佛波醇12-肉豆蔻酸酯13-乙酸酯是U937细胞分化的有效诱导剂，在24小时引起VPF mRNA水平增加12.5倍，与这些单核细胞样细胞分化成巨噬细胞样细胞一致。
Vascular permeability factor (VPF) is an approximately 40-kDa disulfide-linked dimeric glycoprotein that is active in increasing blood vessel permeability, endothelial cell growth and angiogenesis. Little is known about VPF gene regulation. In this study, we investigated the effects of a variety of cytokines and inducing agents on VPF mRNA levels in the monocyte-like U937 cell line. Transforming growth factor-beta 1 caused a 1.8-fold increase in VPF mRNA levels after 4 hours, followed by a decline to basal levels by 18 hours. Phorbol 12-myristate 13-acetate, a potent inducer of the differentiation of U937 cells, caused a 12.5-fold increase in VPF mRNA levels at 24 hours, coinciding with the differentiation of these monocyte-like cells into macrophage-like cells.