Optimization of ultrafiltration/diafiltration processes for partially bound impurities
Optimization of ultrafiltration/diafiltration processes for partially bound impurities
复制标题
优化部分结合杂质的超滤/渗滤工艺
DOI：
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发表时间：
2004
影响因子：
3.8
通讯作者：
A. Zydney
中科院分区：
文献类型：
--
作者：
J. Shao;A. Zydney
超滤和渗滤工艺广泛用于从生物制品中去除各种小杂质。然而，在这些过程中，没有实验或理论分析杂质-产物结合对杂质去除速率的影响。进行模型计算，以解释小杂质和大（保留）产物之间的平衡结合对杂质清除的影响。使用D-色氨酸和牛血清白蛋白作为模型系统进行实验。结果清楚地表明，结合相互作用可以显着降低小杂质的去除率，导致所需的渗滤体积数大幅增加。在存在强结合相互作用的情况下，进行渗滤的最佳产品浓度变为较低的产品浓度。得到了超滤/渗滤过程中杂质去除率的近似解析表达式，为工业超滤/渗滤过程的设计和优化提供了指导。© 2004 Wiley Periodicals，Inc.
Ultrafiltration and diafiltration processes are used extensively for removal of a variety of small impurities from biological products. There has, however, been no experimental or theoretical analysis of the effects of impurity— product binding on the rate of impurity removal during these processes. Model calculations were performed to account for the effects of equilibrium binding between a small impurity and a large (retained) product on impurity clearance. Experiments were performed using D‐tryptophan and bovine serum albumin as a model system. The results clearly demonstrate that binding interactions can dramatically reduce the rate of small impurity removal, leading to large increases in the required number of diavolumes. The optimal product concentration for performing the diafiltration shifts to lower product concentrations in the presence of strong binding interactions. Approximate analytical expressions for the impurity removal were developed which can provide a guide for the design and optimization of industrial ultrafiltration/diafiltration processes. © 2004 Wiley Periodicals, Inc.
影响因子：
4.7
作者：
Falsey, JR;Renil, M;Lam, KS
通讯作者：
Lam, KS