Cortisol is secreted episodically by normal man.
Cortisol is secreted episodically by normal man.
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正常人会偶尔分泌皮质醇。
DOI：
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发表时间：
1970
影响因子：
5.8
通讯作者：
T. Gallagher
中科院分区：
文献类型：
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作者：
L. Hellman;F. Nakada;J. Curti;E. Weitzman;J. Kream;H. Roffwarg;S. Ellman;D. Fukushima;T. Gallagher
一名正常人在0200小时睡眠时接受皮质醇-4-14C。45分钟后，通过使用载体添加和重结晶以恒定同位素比的双同位素衍生分析，频繁地测量血浆皮质醇的比活度（cpm/μg）。还通过竞争性蛋白结合分析和使用3 H-皮质醇的放射化学技术对相同血浆样品的不同部分测量皮质醇水平，以检查操作损失。还对蛋白结合法和放射化学法进行了单独比较。结果表明，通过这些方法获得了令人满意的一致性。结果表明，血浆皮质醇的升高伴随着皮质醇比活度（cpm/μg）的下降。血浆皮质醇水平的福尔斯下降与比活度不变有关。结果表明，在清晨，在睡眠中皮质醇是以阵发性爆发的形式分泌的，这些爆发被没有皮质醇分泌的间隔所分隔。
ABSTRACT A normal man received cortisol-4-14C at 0200 hr while he was asleep. After 45 min the specific activity (cpm/μg) of the plasma cortisol was measured at frequent intervals by double isotope derivative analysis using carrier addition and recrystallization to constant isotope ratio. Cortisol levels were also measured on separate portions of the same plasma sample by competitive protein binding analysis and by radiochemical techniques using 3H-cortisol to check manipulative loss. A separate comparison of protein binding and radiochemical methods was also made. It was shown that satisfactory agreement was obtained by these methods. It was shown that rises in plasma cortisol were accompanied by drops in cortisol specific activity (cpm/μg). Falls in plasma cortisol level were associated with an unchanged specific activity. The results demonstrated that in the early morning hours during sleep cortisol was secreted in episodic bursts which were separated by intervals during which there was no cortisol sec...