Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry
Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry
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DOI：
10.1002/rcm.4579
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发表时间：
2010-06-01
影响因子：
2
通讯作者：
Sang, Shengmin
中科院分区：
文献类型：
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作者：
Shao, Xi;Chen, Xiaoxin;Sang, Shengmin
紫檀芪是白藜芦醇的二甲氧基衍生物，由于其潜在的有益健康作用，最近引起了人们的广泛关注。然而，紫檀芪的代谢命运尚不清楚。在本研究中，我们鉴定了9种新的小鼠尿液中紫檀芪代谢产物：紫檀芪葡萄糖醛酸、硫酸紫檀芪、单去甲基化紫檀芪葡萄糖醛酸、单去甲基化紫檀芪硫酸、单羟基化紫檀芪、单羟基化紫檀芪葡萄糖醛酸、单羟基化紫檀芪硫酸、单羟基化紫檀芪硫酸、单羟基化紫檀芪硫酸、单羟基化紫檀芪硫酸葡萄糖醛酸。采用液相色谱/常压化学电离和电喷雾电离串联质谱法。这些代谢物的结构通过MSn （n = 1-3）谱分析得到了证实。据我们所知，这是第一个在小鼠尿液中鉴定紫檀芪代谢产物的报告。版权所有John Wiley & Sons， Ltd。
Pterostilbene, the dimethoxy derivative of resveratrol, has drawn much attention recently due to its potential beneficial health effects. The metabolic fate of pterostilbene, however, is not well understood. In the present study, we identified nine novel mouse urinary pterostilbene metabolites, pterostilbene glucuronide, pterostilbene sulfate, mono-demethylated pterostilbene glucuronide, mono-demethylated pterostilbene sulfate, mono-hydroxylated pterostilbene, mono-hydroxylated pterostilbene glucuronide, mono-hydroxylated pterostilbene sulfate, and mono-hydroxylated pterostilbene glucuronide sulfate, using liquid chromatography/atmospheric pressure chemical ionization and electrospray ionization tandem mass spectrometry. The structures of these metabolites were confirmed by analyzing the MSn (n = 1-3) spectra. To our knowledge, this is the first report of the identification of urinary metabolites of pterostilbene in mice. Copyright (C) 2010 John Wiley & Sons, Ltd.