The Protective Effect of Grape-Seed Proanthocyanidin Extract on Oxidative Damage Induced by Zearalenone in Kunming Mice Liver.
The Protective Effect of Grape-Seed Proanthocyanidin Extract on Oxidative Damage Induced by Zearalenone in Kunming Mice Liver.
复制标题
葡萄籽原花青素提取物对玉米赤霉烯酮所致昆明小鼠肝脏氧化损伤的保护作用
DOI：
10.3390/ijms17060808
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发表时间：
2016-05-25
影响因子：
5.6
通讯作者：
He JB
中科院分区：
文献类型：
--
作者：
Long M;Yang SH;Han JX;Li P;Zhang Y;Dong S;Chen X;Guo J;Wang J;He JB
虽然葡萄籽原花青素提取物（GSPE）显示出很强的抗氧化活性，但很少有研究清楚地揭示了玉米赤霉烯酮（ZEN）引起的肝毒性的保护作用。本研究旨在探讨GSPE对ZEN诱导的小鼠肝脏氧化损伤的保护作用及其可能的分子机制。结果表明，GSPE能显著降低ZEN引起的血清谷草转氨酶（AST）和丙氨酸转氨酶（ALT）活性升高。GSPE还能显著降低MDA含量，提高抗氧化酶SOD和GSH-Px活性。分析表明，ZEN降低核红细胞2相关因子2（Nrf 2）的mRNA表达水平和蛋白表达水平。Nrf 2被认为是一种重要的抗氧化转录因子，因为下游GSH-Px、γ-谷氨酰半胱氨酸合成酶（γ-GCS）、血红素加氧酶-1（HO-1）和醌氧化还原酶1（NQO 1）同时降低，而预先给予GSPE组显示这些表达升高。结果表明，GSPE对ZEN诱导的肝损伤具有保护作用，其机制可能与激活Nrf 2/ARE信号通路有关。
Although grape-seed proanthocyanidin extract (GSPE) demonstrates strong anti-oxidant activity, little research has been done to clearly reveal the protective effects on the hepatotoxicity caused by zearalenone (ZEN). This study is to explore the protective effect of GSPE on ZEN-induced oxidative damage of liver in Kunming mice and the possible protective molecular mechanism of GSPE. The results indicated that GSPE could greatly reduce the ZEN-induced increase of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities. GSPE also significantly decreased the content of MDA but enhanced the activities of antioxidant enzymes SOD and GSH-Px. The analysis indicated that ZEN decreased both mRNA expression levels and protein expression levels of nuclear erythroid2-related factor2 (Nrf2). Nrf2 is considered to be an essential antioxidative transcription factor, as downstream GSH-Px, γ-glutamyl cysteine synthetase (γ-GCS), hemeoxygenase-1 (HO-1), and quinone oxidoreductase 1 (NQO1) decreased simultaneously, whereas the pre-administration of GSPE groups was shown to elevate these expressions. The results indicated that GSPE exerted a protective effect on ZEN-induced hepatic injury and the mechanism might be related to the activation of the Nrf2/ARE signaling pathway.