Quantitative molecular characterization of bovine vitreous and lens with non-invasive dynamic light scattering
Quantitative molecular characterization of bovine vitreous and lens with non-invasive dynamic light scattering
复制标题
DOI：
10.1006/exer.2001.1097
复制
发表时间：
2001-12-01
影响因子：
3.4
通讯作者：
Sebag, J
中科院分区：
文献类型：
--
作者：
Ansari, RR;Suh, KI;Sebag, J
非侵入性动态光散射(DLS)技术通过测量主要颗粒的尺寸并绘制这些结构大分子在三维空间的三维形貌分布，在分子水平上定量表征玻璃体和晶状体的结构。将5只新鲜成年牛眼的DLS测量结果与透明质酸(HA)和胶原(Coll)模型溶液中的DLS测量结果进行了比较。在牛眼中，从凝胶和液体玻璃体的切除样本中获得了DLS测量，并与模型溶液进行了比较。整个玻璃体的测量是在晶状体后面的多个点上进行的，以生成分子结构的三维“地图”。牛晶状体中的大分子分布也是相似的。在每个牛玻璃体(Bo Vit)标本中，DLS主要检测到两个不同的颗粒，它们的扩散性质和大小不同。与模型玻璃体溶液的比较表明，这些最可能与玻璃体的Coll和HA组分相对应。Bo Vit的三维成像发现玻璃体内的异质性，在不同的部位Coll和HA的颗粒大小分布不同。相反，晶状体大分子的三维分布更均匀。因此，无创DLS技术可以定量测量玻璃体和晶状体大分子的平均大小，并绘制其三维分布图。这种定量评估玻璃体和晶状体大分子结构的方法，对临床和实验应用于健康和疾病都是有用的。(C)2001年学术出版社。
The non-invasive technique of dynamic light scattering (DLS) was used to quantitatively characterize vitreous and lens structure on a molecular level by measuring the sizes of the predominant particles and mapping the three-dimensional topographic distribution of these structural macromolecules in three spatial dimensions. The results of DLS measurements in five fresh adult bovine eyes were compared to DLS measurements in model solutions of hyaluronan (HA) and collagen (Coll). In the bovine eyes DLS measurements were obtained from excised samples of gel and liquid vitreous and compared to the model solutions. Measurements in whole vitreous were obtained at: multiple points posterior to the lens to generate a three-dimensional 'map' of molecular structure. The macromolecule distribution in bovine lens was similarly characterized.In each bovine vitreous (Bo Vit) specimen, DLS predominantly detected two distinct particles, which differed in diffusion properties and hence size. Comparisons with model vitreous solutions demonstrated that these most likely corresponded to the Coll and HA components of vitreous. Three-dimensional mapping of Bo Vit found heterogeneity, throughout the vitreous body, with different particle size distributions for Coll and HA at different loci. In contrast, the three-dimensional distribution of lens macromolecules was more homogeneous. Thus, the non-invasive DLS technique can quantitate the average sizes of vitreous and lens macromolecules and map their three-dimensional distribution. This method to assess quantitatively the inacromolecular structure of vitreous and lens should be useful for clinical as well as experimental applications in health and disease. (C) 2001 Academic Press.